Centro Tecnológico de la Carne de Galicia, Rúa Galicia No. 4, Parque Tecnológico de Galicia, San Cibrán das Viñas, 32900 Ourense, Spain.
Meat Sci. 2014 Jan;96(1):179-86. doi: 10.1016/j.meatsci.2013.06.017. Epub 2013 Jun 15.
Extraction of dry-cured loin volatile compounds by solid-phase microextraction (SPME) was optimized. Two different fiber coatings: carboxen/polydimethylsiloxane (CAR/PDMS) and divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS), and three extraction times (15, 30 and 45 min) were assayed. Between the fibers tested, CAR/PDMS coated fibers extracted between two and three times more total amount of volatile compounds than the DVD/CAR/PDMS coating (1314×10(6) vs. 526×10(6) area units). Fifty five compounds were extracted by the CAR/PDMS fiber, while only 48 of these were found with the DVB/CAR/PDMS fiber. On the other hand, 6 additional volatile compounds were extracted by the CAR/PDMS coating, all of them being of low molecular weight. Three of the major compounds extracted, hexanoic acid, methyl ester; butanoic acid, 3-methyl-, methyl ester and hexanal, were found in high proportion in both fiber coatings. The effect of exposure time was more marked for the DVD/CAR/PDMS fiber than for the CAR/PDMS coating. Fifteen minutes of extraction provided a volatile compounds profile with lower area counts for most compounds and significantly different to that obtained with 30 and 45 min of extraction. The extraction yields of dry-cured loin volatile compounds varied according to the fiber coating used and the time of exposure. Therefore, extraction conditions should be selected depending on the objective of the study. Finally, it can be concluded that both porous fibers tested, CAR/PDMS and DVB/CAR/PDMS, provide a similar volatile compounds profile for dry-cured foal loin.
采用固相微萃取（SPME）对干腌里脊中的挥发性化合物进行提取，并对其进行了优化。试验了两种不同的纤维涂层：CAR/PDMS（羧基/聚二甲基硅氧烷）和 DVB/CAR/PDMS（二乙烯基苯/羧基/聚二甲基硅氧烷），以及三种萃取时间（15、30 和 45 min）。在测试的纤维中，CAR/PDMS 涂层纤维比 DVB/CAR/PDMS 涂层纤维萃取的挥发性化合物总量多 2 到 3 倍（1314×10(6)与 526×10(6)面积单位）。CAR/PDMS 纤维萃取了 55 种化合物，而 DVB/CAR/PDMS 纤维只发现了 48 种。另一方面，CAR/PDMS 涂层萃取了 6 种额外的挥发性化合物，它们都是低分子量化合物。从两种纤维涂层中萃取的三种主要化合物是己酸甲酯、丁酸-3-甲基甲酯和己醛，它们在两种纤维涂层中都占有较大的比例。暴露时间对 DVB/CAR/PDMS 纤维的影响比对 CAR/PDMS 涂层的影响更为显著。15 分钟的萃取时间提供了一个挥发性化合物图谱，图谱中大多数化合物的面积计数较低，与 30 和 45 分钟萃取得到的图谱有显著差异。干腌里脊挥发性化合物的萃取产率取决于所使用的纤维涂层和暴露时间。因此，应根据研究的目的选择萃取条件。最后，可以得出结论，两种多孔纤维，CAR/PDMS 和 DVB/CAR/PDMS，为干腌马肉里脊提供了相似的挥发性化合物图谱。
