Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Nat Commun. 2013;4:2215. doi: 10.1038/ncomms3215.
Plants control CO2 uptake and water loss by modulating the aperture of stomata located in the epidermis. Stomatal opening is initiated by the activation of H(+)-ATPases in the guard-cell plasma membrane. In contrast to regulation of H(+)-ATPase activity, little is known about the translocation of the guard cell H(+)-ATPase to the plasma membrane. Here we describe the isolation of an Arabidopsis gene, PATROL1, that controls the translocation of a major H(+)-ATPase, AHA1, to the plasma membrane. PATROL1 encodes a protein with a MUN domain, known to mediate synaptic priming in neuronal exocytosis in animals. Environmental stimuli change the localization of plasma membrane-associated PATROL1 to an intracellular compartment. Plasma membrane localization of AHA1 and stomatal opening require the association of PATROL1 with AHA1. Increased stomatal opening responses in plants overexpressing PATROL1 enhance the CO2 assimilation rate, promoting plant growth.
植物通过调节位于表皮中的气孔的孔径来控制 CO2 的摄取和水分的损失。气孔的开启是由保卫细胞质膜中的 H(+)-ATP 酶的激活引发的。与 H(+)-ATP 酶活性的调节相比，对于保卫细胞 H(+)-ATP 酶向质膜的易位知之甚少。在这里，我们描述了拟南芥基因 PATROL1 的分离，该基因控制主要的 H(+)-ATP 酶 AHA1 向质膜的易位。PATROL1 编码一种具有 MUN 结构域的蛋白质，该结构域已知在动物的神经元胞吐作用中介导突触启动。环境刺激会改变与质膜相关的 PATROL1 的定位到细胞内隔室。AHA1 的质膜定位和气孔的开启需要 PATROL1 与 AHA1 的结合。过表达 PATROL1 的植物的气孔开度反应增加，提高了 CO2 同化率，促进了植物的生长。
