Imamura N, Mtasiwa D M, Ota H, Inada T, Kuramoto A
Department of Internal Medicine, Hiroshima University, Japan.
Am J Hematol. 1990 Sep;35(1):65-7. doi: 10.1002/ajh.2830350115.
We herein describe CD9 (p24) antigen as existing on the cell surface of megakaryocyte lineage leukemias as well as megakaryocytic leukemia cell line, MEG-01 and HEL, by means of fluorescence-activated cell sorter (FACS IV) with a panel of monoclonal antibodies (Mabs). We found CD9 antigen expression on the cell surface of megakaryoblastic leukemias as well as MEG-01 and HEL cells. Furthermore, CD9 antigen expression increased while culturing these cells with phorbol esters, and was also found in the cytoplasm by means of indirect immunofluorescence test. These findings clearly showed that CD9 antigen exists on the cell surface of megakaryocytic cells which are capable of synthesizing the CD9.
我们在此借助荧光激活细胞分选仪（FACS IV）及一组单克隆抗体（Mabs），描述了CD9（p24）抗原存在于巨核细胞系白血病以及巨核细胞白血病细胞系MEG - 01和HEL的细胞表面。我们发现CD9抗原在巨核母细胞白血病以及MEG - 01和HEL细胞的细胞表面表达。此外，在用佛波酯培养这些细胞时，CD9抗原表达增加，并且通过间接免疫荧光试验在细胞质中也发现了该抗原。这些发现清楚地表明，CD9抗原存在于能够合成CD9的巨核细胞的细胞表面。
