Hindegade 5, 4. tv., 1303 København K, Denmark.
Anticancer Res. 2013 Aug;33(8):3033-9.
BACKGROUND/AIM: With increasing incidence of non-melanoma skin cancer (NMSC), focus on chemoprevention of this disease is growing. The aim of this study was to evaluate topical combination therapies as chemoprevention of UV radiation-induced tumors in a mouse model.
A total of 160 SKH-1 mice were randomized to one placebo group and four chemoprevention groups (diclofenac plus difluoromethylornithine; diclofenac plus calcipotriol; difluoromethylornithine plus calcitriol; and diclofenac plus difluoromethylornithine plus calcipotriol). The mice received UVB radiation for 20 weeks followed by 17 weeks with topical application of chemoprevention. The number of mice with tumors, number of tumors per group and tumor area size were compared using a linear regression model.
Chemoprevention with diclonefac plus calcipotriol and diclonefac plus difluoromethylornithine had a significant inhibiting effect on the number of tumors per group and the area of tumors. Moreover, diclonefac plus difluoromethylornithine had a significant inhibiting effect on the number of mice with tumors.
Potentially, non-melanoma skin cancer in humans may be prevented with these agents with few adverse effects. Therefore, clinical studies are needed to determine their therapeutic/preventive effect and possible adverse effects.
背景/目的：随着非黑素瘤皮肤癌（NMSC）发病率的增加，人们对这种疾病的化学预防越来越关注。本研究旨在评估局部联合治疗作为一种预防 UV 辐射诱导的肿瘤的方法。
总共 160 只 SKH-1 小鼠被随机分为一组安慰剂组和四组化学预防组（双氯芬酸加二氟甲基鸟氨酸；双氯芬酸加钙泊三醇；二氟甲基鸟氨酸加钙三醇；双氯芬酸加二氟甲基鸟氨酸加钙泊三醇）。这些小鼠接受了 20 周的 UVB 辐射，然后进行了 17 周的化学预防局部治疗。使用线性回归模型比较每组小鼠的肿瘤数量、肿瘤数量和肿瘤面积大小。
双氯芬酸加钙泊三醇和双氯芬酸加二氟甲基鸟氨酸的化学预防对每组肿瘤数量和肿瘤面积具有显著抑制作用。此外，双氯芬酸加二氟甲基鸟氨酸对患肿瘤的小鼠数量具有显著抑制作用。
这些药物可能具有预防人类非黑素瘤皮肤癌的潜力，且不良反应较少。因此，需要进行临床研究以确定其治疗/预防效果和可能的不良反应。
