Departments of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Phatumwan, Bangkok, Thailand 10330.
Anticancer Res. 2013 Aug;33(8):3079-88.
Knowledge regarding substances that attenuate motility of cancer cells has gathered significant attention, as they benefit the development of novel anticancer strategies. The anti-migration and anti-invasion activities of artonin E, extracted from bark of Artocarpus gomezianus, were investigated in lung cancer cells in this study.
Cytotoxicity and antiproliferative effects of artonin E were examined by 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Migration and invasion assays were performed on H460, H23, A549 and H292 human lung cancer cells. Cell morphology was determined by phalloidin-rhodamine staining. Motility-related proteins were investigated by western blotting.
Artonin E exhibited anti-migration and anti-invasion activities in H460 cells. Cell morphology revealed that treatment of the cells with non-toxic concentrations of artonin E resulted in a decrease of activated focal adhesion kinase (FAK), downstream protein kinase B (AKT) activation, and Cell division cycle-42 (CDC42), all of which were associated with the anti-motility effect of this compound. Artonin E inhibited invasion and migration of other lung cancer cells, namely H292, H23 and A549 cells.
These results suggest that artonin E may be a promising candidate for anti-metastasis use.
具有抑制癌细胞运动能力的物质已引起广泛关注，因为它们有助于开发新的抗癌策略。本研究旨在探讨从 Artocarpus gomezianus 树皮中提取的 artonin E 对肺癌细胞的抗迁移和抗侵袭活性。
采用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法检测 artonin E 的细胞毒性和抗增殖作用。在 H460、H23、A549 和 H292 人肺癌细胞中进行迁移和侵袭实验。通过鬼笔环肽-rhodamine 染色观察细胞形态。采用 Western blot 法检测与细胞迁移相关的蛋白。
Artonin E 对 H460 细胞表现出抗迁移和抗侵袭活性。细胞形态学观察表明，用无毒浓度的 artonin E 处理细胞可导致激活的粘着斑激酶（FAK）、下游蛋白激酶 B（AKT）的激活以及细胞分裂周期蛋白 42（CDC42）减少，所有这些都与该化合物的抗运动能力有关。Artonin E 还抑制了其他肺癌细胞（即 H292、H23 和 A549 细胞）的侵袭和迁移。
这些结果表明，Artonin E 可能是一种有前途的抗转移候选药物。
