miR-146a 多态性（rs2910164）可预测结直肠癌的发病风险和生存情况。

A miR-146a polymorphism (rs2910164) predicts risk of and survival from colorectal cancer.

机构信息

Kyungpook National University Medical Center, Kyungpook National University School of Medicine, 807 Hogukno, Buk-Gu, Daegu 702-210, Korea.

出版信息

Anticancer Res. 2013 Aug;33(8):3233-9.

PMID:23898084

Abstract

BACKGROUND

Recent evidence suggests that the rs2910164 variant of miR-146a is associated with the development of certain types of cancer. Therefore, the aim of this study was to investigate the association of this genetic variant with susceptibility and prognosis in patients with colorectal cancer (CRC).

MATERIALS AND METHODS

Genotyping analyses of miR-146a rs2690164 for risk and survival in CRC were performed in a case-control study (n=967) using a polymerase chain reaction (PCR)-restriction fragment length polymorphism assay.

RESULTS

The C allelic frequency of miR-146 rs2690164 in the 399 patients and 568 controls was 61.9% and 53.9%, respectively. In the case-control study, those who possessed the CC genotype had a higher risk of CRC compared to those with the CG or GG genotype (odds ratio=1.569; 95% confidence interval=1.196-2.059; p=0.001), regardless of the tumor site. In the survival analysis of 343 patients with CRC who underwent curative surgery, those with CC genotype had a worse survival outcome compared with those with CG or GG genotype in a Kaplan-Meier survival analysis. Moreover, a multivariate analysis showed that the CC genotype of miR-146a rs2910164 was associated with worse relapse-free and disease-specific survival compared to the CG or GG genotype in a recessive model of the C allele, adjusted for patient and tumor characteristics (hazard ratio=2.120 and 2.349, p=0.005 and 0.007, respectively).

CONCLUSION

The current study provides evidence that the miR-146a rs2690164 polymorphism, as the dominant model of the G allele, is associated with the susceptibility and prognosis of CRC.

摘要

背景

最近的证据表明，miR-146a 的 rs2910164 变体与某些类型的癌症的发展有关。因此，本研究旨在探讨这种遗传变异与结直肠癌（CRC）患者易感性和预后的关系。

材料和方法

采用聚合酶链反应（PCR）-限制性片段长度多态性分析，对 miR-146a rs2690164 进行基因分型分析，以评估其在 CRC 中的风险和生存情况。在一项病例对照研究（n=967）中，我们使用聚合酶链反应（PCR）-限制性片段长度多态性分析方法，对 miR-146a rs2690164 的基因型进行了分析。

结果

在 399 例患者和 568 例对照中，miR-146 rs2690164 的 C 等位基因频率分别为 61.9%和 53.9%。在病例对照研究中，与 CG 或 GG 基因型相比，携带 CC 基因型的患者患 CRC 的风险更高（比值比=1.569；95%置信区间=1.196-2.059；p=0.001），且与肿瘤部位无关。在对 343 例接受根治性手术的 CRC 患者进行生存分析时，Kaplan-Meier 生存分析显示，CC 基因型患者的生存结局较 CG 或 GG 基因型患者差。此外，多变量分析显示，在隐性 C 等位基因模型中，与 CG 或 GG 基因型相比，miR-146a rs2910164 的 CC 基因型与无复发生存和疾病特异性生存较差相关，调整了患者和肿瘤特征（风险比=2.120 和 2.349，p=0.005 和 0.007）。