Interactions Arbres/Micro-organismes, Faculté des Sciences, UMR1136 Université de Lorraine-INRA Vandoeuvre, France.
Front Plant Sci. 2013 Jul 24;4:259. doi: 10.3389/fpls.2013.00259. eCollection 2013.
Many metabolic pathways and cellular processes occurring in most sub-cellular compartments depend on the functioning of iron-sulfur (Fe-S) proteins, whose cofactors are assembled through dedicated protein machineries. Recent advances have been made in the knowledge of the functions of individual components through a combination of genetic, biochemical and structural approaches, primarily in prokaryotes and non-plant eukaryotes. Whereas most of the components of these machineries are conserved between kingdoms, their complexity is likely increased in plants owing to the presence of additional assembly proteins and to the existence of expanded families for several assembly proteins. This review focuses on the new actors discovered in the past few years, such as glutaredoxin, BOLA and NEET proteins as well as MIP18, MMS19, TAH18, DRE2 for the cytosolic machinery, which are integrated into a model for the plant Fe-S cluster biogenesis systems. It also discusses a few issues currently subjected to an intense debate such as the role of the mitochondrial frataxin and of glutaredoxins, the functional separation between scaffold, carrier and iron-delivery proteins and the crosstalk existing between different organelles.
许多发生在大多数亚细胞区室中的代谢途径和细胞过程依赖于铁硫（Fe-S）蛋白的功能，其辅因子通过专门的蛋白质机器组装。通过遗传、生化和结构方法的结合，主要在原核生物和非植物真核生物中，对单个组件的功能的认识已经取得了进展。尽管这些机器的大多数组件在各个领域中都得到了保守，但由于存在额外的组装蛋白以及几个组装蛋白的扩展家族，它们的复杂性可能在植物中增加。本文综述了过去几年中发现的新因子，如谷氧还蛋白、BOLA 和 NEET 蛋白以及细胞质机器中的 MIP18、MMS19、TAH18 和 DRE2，它们被整合到植物 Fe-S 簇生物发生系统的模型中。它还讨论了目前正在激烈争论的几个问题，如线粒体 frataxin 和谷氧还蛋白的作用、支架、载体和铁传递蛋白之间的功能分离以及不同细胞器之间的串扰。
