Nano- & Micro-Fluidics Team, Industrial Research Ltd., 69 Gracefield Road, P.O. Box 31310, Lower Hutt 5040, New Zealand.
Biomicrofluidics. 2012 Jul 30;6(3):36503. doi: 10.1063/1.4740232. Print 2012 Sep.
A rapid, inexpensive method using alkoxysilanes has been developed to selectively coat the interior of polydimethylsiloxane (PDMS) microfluidic channels with an integral silicaceous layer. This method combines the rapid prototyping capabilities of PDMS with the desirable wetting and electroosmotic properties of glass. The procedure can be carried out on the open faces of PDMS blocks prior to enclosure of the channels, or by flowing the reagents through the preformed channels. Therefore, this methodology allows for high-throughput processing of entire microfluidic devices or selective modification of specific areas of a device. Modification of PDMS with tetraethoxysilane generated a stable surface layer, with enhanced wettability and a more stable electroosmotic flow rate than native PDMS. Modification of PDMS with 3-aminopropyltriethoxysilane generated a surface layer bearing amine functionalities allowing for further chemical derivatization of the PDMS surface.
已经开发出一种使用烷氧基硅烷的快速、廉价的方法，可选择性地在聚二甲基硅氧烷 (PDMS) 微流道的内部涂覆一层整体的硅质层。该方法将 PDMS 的快速原型制造能力与玻璃的理想润湿性和电动渗透性相结合。该过程可以在通道封闭之前在 PDMS 块的开口面上进行，或者通过使试剂流过预先形成的通道进行。因此，该方法允许对整个微流控器件进行高通量处理，或者对器件的特定区域进行选择性修饰。用四乙氧基硅烷对 PDMS 进行修饰会生成稳定的表面层，与天然 PDMS 相比，其润湿性增强，电动渗透率更稳定。用 3-氨丙基三乙氧基硅烷对 PDMS 进行修饰会生成带有胺官能团的表面层，从而允许对 PDMS 表面进行进一步的化学衍生化。
