Cakina Suat, Gulyasar Tevfik, Ozen Alaattin, Sipahi Tammam, Kocak Zafer, Sener Seralp
Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey.
Indian J Biochem Biophys. 2013 Jun;50(3):233-6.
The roles of many genes in the pathophysiology of lung cancer have been investigated in different studies. Cyclin D1 (CCND1) gene plays a significant role in the transition from G1 to S phase of the cell cycle and in the phosphorylation of retinoblastoma tumor suppressor protein. In this study, we aimed to identify the relationship between CCND1 A870G gene polymorphism with lung cancer. CCND1 A870G genotypes were determined in 75 patients with lung cancer and in 65 control subjects. DNA was isolated from blood samples and then CCND1 A870G gene polymorphism was identified using PCR and RFLP assay. The distribution of CCND1 A870G polymorphism did not show any significant differences in all lung cancer patients and controls. There was no correlation between CCND1 A870G polymorphism and histopathological findings. However, the AA + AG genotype was significantly higher in metastatic patients, when compared with non-metastatic patients. Thus, the results show that CCND1 gene polymorphism may be a predictor for detecting patients with poor survival who having metastatic disease.
在不同的研究中，已经对许多基因在肺癌病理生理学中的作用进行了调查。细胞周期蛋白D1（CCND1）基因在细胞周期从G1期到S期的转变以及视网膜母细胞瘤肿瘤抑制蛋白的磷酸化过程中发挥着重要作用。在本研究中，我们旨在确定CCND1 A870G基因多态性与肺癌之间的关系。在75例肺癌患者和65例对照受试者中测定了CCND1 A870G基因型。从血液样本中分离DNA，然后使用PCR和RFLP分析鉴定CCND1 A870G基因多态性。CCND1 A870G多态性的分布在所有肺癌患者和对照中均未显示出任何显著差异。CCND1 A870G多态性与组织病理学结果之间没有相关性。然而，与非转移性患者相比，转移性患者中AA + AG基因型显著更高。因此，结果表明CCND1基因多态性可能是检测患有转移性疾病的生存不良患者的一个预测指标。
