Department of Pulmonary and Critical Care Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C.
Anticancer Res. 2011 Oct;31(10):3601-5.
Cyclin D1 (CCND1) is critical in the transition of the cell cycle from G1 to S phase and unbalanced cell cycle regulation is a hallmark of carcinogenesis. The study aimed at investigating the association of CCND1 genotypes with lung cancer risk in Taiwan and examining the interaction between CCND1 genotype and smoking habit.
CCND1 A870G (rs9344) and C1722G (rs678653) genotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of DNA from the blood of 358 lung cancer patients and 716 cancer-free healthy controls.
The results showed that there were significant differences between lung cancer and control groups in the distribution of the genotypes (p=0.0003) and allelic frequency (p=0.0007) in the CCND1 rs9344 genotype. Individuals who carried AG or GG genotype had 0.59- and 0.52-fold risk, respectively, of developing lung cancer compared to those who carried the AA genotype (95% CI=0.44-0.78 and 0.35-0.79, respectively). There was also an obvious interaction of CCND1 rs9344 genotype with personal smoking habit on lung cancer risk (p=0.0009).
These findings support the conclusion that cell cycle regulation may play a role in lung cancer development and that CCND1 rs9344 polymorphism together with smoking habit maybe a useful biomarker for lung cancer prediction.
细胞周期蛋白 D1（CCND1）在细胞周期从 G1 期到 S 期的过渡中起着关键作用，细胞周期调控失衡是致癌作用的标志。本研究旨在探讨 CCND1 基因型与台湾地区肺癌风险的关系，并研究 CCND1 基因型与吸烟习惯之间的相互作用。
通过聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析，检测了 358 例肺癌患者和 716 例无癌健康对照者血液中的 CCND1 A870G（rs9344）和 C1722G（rs678653）基因型。
结果表明，CCND1 rs9344 基因型的分布在肺癌组和对照组之间存在显著差异（p=0.0003）和等位基因频率（p=0.0007）。与携带 AA 基因型的个体相比，携带 AG 或 GG 基因型的个体患肺癌的风险分别增加了 0.59-和 0.52 倍（95%CI=0.44-0.78 和 0.35-0.79）。CCND1 rs9344 基因型与个人吸烟习惯对肺癌风险也存在明显的交互作用（p=0.0009）。
这些发现支持这样的结论，即细胞周期调控可能在肺癌的发生发展中起作用，CCND1 rs9344 多态性与吸烟习惯相结合可能是肺癌预测的有用生物标志物。
