Deng Xiao-Ming, Luo Xiao-Jia, Chen Xiao-Ping, He Sen, Li Long-Xin, Wan Li-Yan, Peng Yong
Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 May;44(3):383-7.
To determine the mRNA expressions of PPARalpha and PPARbeta in peripheral blood mononuclear cells of non-vavular hypertensive atrial fibrillation (AF) patients and elucidate its possible role in the pathogenesis of AF.
Peripheral blood samples were collected from 103 patients with hypertensive AF (persistent AF: 55, paroxysmal AF: 48) and 50 age-adjusted hypertension patients without AF. The mRNA expressions of PPARalpha, PPARbeta, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in monocytes were detected by using a Real time polymerase chain reaction. The concentrations of high sensitive C-reactive protein (CRP) and interleukin-1 (IL-1) were measured by immunoenzymetric method.
The PPARalpha mRNA expression level was persistently decreased in hypertensive non-AF group, paroxysmal AF group, and persistent AF group (1.34 +/- 0.17, 1.09 +/- 0.23, 0.85 +/- 0.22), while the difference was statistically significant (P < 0.001; respectively). TNF-alpha mRNA, IL-6 mRNA,CRP and IL-1 persistently increased in hypertensive non-AF group, paroxysmal AF group, persistent AF group, also the difference was statistically significant (P < 0. 001; respectively). The difference of PPARbeta mRNA was not statistically significant between non-AF group, paroxysmal AF group and persistent AF group. Left atrial diameter (LAD) was in positive correlation with CRP, IL-1, IL-6 mRNA and TNF-alpha mRNA (P < 0.05). PPARalpha mRNA level was in negative correlation with CRP, IL-1, IL-6 mRNA and TNF-alpha mRNA, the correlation coefficient was -0.519, -0.532, -0.491 and -0.528, respectively (P < 0.05).
In hypertensive patients with AF, increased inflammatory cytokines were associated with atrial remodeling and lead to the development of atrial fibrillation; PPARalpha was negatively correlated with these inflammatory cytokines and may play a vital role in the process of atrial fibrillation development.
测定非瓣膜性高血压心房颤动（AF）患者外周血单个核细胞中PPARα和PPARβ的mRNA表达，并阐明其在AF发病机制中的可能作用。
收集103例高血压AF患者（持续性AF：55例，阵发性AF：48例）及50例年龄匹配的无AF高血压患者的外周血样本。采用实时聚合酶链反应检测单核细胞中PPARα、PPARβ、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的mRNA表达。采用免疫酶法测定高敏C反应蛋白（CRP）和白细胞介素-1（IL-1）的浓度。
高血压非AF组、阵发性AF组和持续性AF组的PPARα mRNA表达水平持续降低（分别为1.34±0.17、1.09±0.23、0.85±0.22），差异有统计学意义（P<0.001）。高血压非AF组、阵发性AF组和持续性AF组的TNF-α mRNA、IL-6 mRNA、CRP和IL-1持续升高，差异有统计学意义（P<0.001）。非AF组、阵发性AF组和持续性AF组之间PPARβ mRNA的差异无统计学意义。左房内径（LAD）与CRP、IL-1、IL-6 mRNA和TNF-α mRNA呈正相关（P<0.05）。PPARα mRNA水平与CRP、IL-1、IL-6 mRNA和TNF-α mRNA呈负相关，相关系数分别为-0.519、-0.532、-0.491和-0.528（P<0.05）。
在高血压AF患者中，炎性细胞因子增加与心房重构相关，并导致心房颤动的发生；PPARα与这些炎性细胞因子呈负相关，可能在心房颤动的发生过程中起重要作用。
