[非瓣膜性高血压心房颤动患者外周血单个核细胞中过氧化物酶体增殖物激活受体α和β的表达]

[Expression of peroxisome proliferator-activated receptor alpha and beta in peripheral blood mononuclear cells of non-vavular hypertensive atrial fibrillation patients].

作者信息

Deng Xiao-Ming, Luo Xiao-Jia, Chen Xiao-Ping, He Sen, Li Long-Xin, Wan Li-Yan, Peng Yong

机构信息

Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 May;44(3):383-7.

PMID:23898518

Abstract

OBJECTIVE

To determine the mRNA expressions of PPARalpha and PPARbeta in peripheral blood mononuclear cells of non-vavular hypertensive atrial fibrillation (AF) patients and elucidate its possible role in the pathogenesis of AF.

METHODS

Peripheral blood samples were collected from 103 patients with hypertensive AF (persistent AF: 55, paroxysmal AF: 48) and 50 age-adjusted hypertension patients without AF. The mRNA expressions of PPARalpha, PPARbeta, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in monocytes were detected by using a Real time polymerase chain reaction. The concentrations of high sensitive C-reactive protein (CRP) and interleukin-1 (IL-1) were measured by immunoenzymetric method.

RESULTS

The PPARalpha mRNA expression level was persistently decreased in hypertensive non-AF group, paroxysmal AF group, and persistent AF group (1.34 +/- 0.17, 1.09 +/- 0.23, 0.85 +/- 0.22), while the difference was statistically significant (P < 0.001; respectively). TNF-alpha mRNA, IL-6 mRNA,CRP and IL-1 persistently increased in hypertensive non-AF group, paroxysmal AF group, persistent AF group, also the difference was statistically significant (P < 0. 001; respectively). The difference of PPARbeta mRNA was not statistically significant between non-AF group, paroxysmal AF group and persistent AF group. Left atrial diameter (LAD) was in positive correlation with CRP, IL-1, IL-6 mRNA and TNF-alpha mRNA (P < 0.05). PPARalpha mRNA level was in negative correlation with CRP, IL-1, IL-6 mRNA and TNF-alpha mRNA, the correlation coefficient was -0.519, -0.532, -0.491 and -0.528, respectively (P < 0.05).

CONCLUSION

In hypertensive patients with AF, increased inflammatory cytokines were associated with atrial remodeling and lead to the development of atrial fibrillation; PPARalpha was negatively correlated with these inflammatory cytokines and may play a vital role in the process of atrial fibrillation development.

摘要

目的

测定非瓣膜性高血压心房颤动（AF）患者外周血单个核细胞中PPARα和PPARβ的mRNA表达，并阐明其在AF发病机制中的可能作用。

方法

收集103例高血压AF患者（持续性AF：55例，阵发性AF：48例）及50例年龄匹配的无AF高血压患者的外周血样本。采用实时聚合酶链反应检测单核细胞中PPARα、PPARβ、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的mRNA表达。采用免疫酶法测定高敏C反应蛋白（CRP）和白细胞介素-1（IL-1）的浓度。

结果

高血压非AF组、阵发性AF组和持续性AF组的PPARα mRNA表达水平持续降低（分别为1.34±0.17、1.09±0.23、0.85±0.22），差异有统计学意义（P<0.001）。高血压非AF组、阵发性AF组和持续性AF组的TNF-α mRNA、IL-6 mRNA、CRP和IL-1持续升高，差异有统计学意义（P<0.001）。非AF组、阵发性AF组和持续性AF组之间PPARβ mRNA的差异无统计学意义。左房内径（LAD）与CRP、IL-1、IL-6 mRNA和TNF-α mRNA呈正相关（P<0.05）。PPARα mRNA水平与CRP、IL-1、IL-6 mRNA和TNF-α mRNA呈负相关，相关系数分别为-0.519、-0.532、-0.491和-0.528（P<0.05）。