Wang Xiao-Hui, Zong Zhi-Yong, Lü Xiao-Ju
Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 May;44(3):405-9.
To investigate the clonal relatedness of local bla(OXA-58)-carrying Acinetobacter baumannii clinical isolates.
Non-duplicated isolates of Acinetobacter baumannii were collected in West China Hospital and verified by recA sequencing. Acquired bla(OXA-58) gene and natural bla(OXA51/66) genes were detected by PCR. Strain typing for bla(OXA-58)-carrying Acinetobacter baumannii isolates was performed by Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE).
A total of 115 Acinetobacter baumannii isolates were verified by recA gene and bla(OXA-51/6)6 detection. Among them, nine (7.8%) isolates carry bla(OXA-58) with reduced susceptibility to imipenem (MIC > or = 2 mg/L) were observed. ERIC-PCR fingerprints of nine bla(OXA-58)-carrying isolates were highly similar. MLST revealed that eight isolates were ST95 and one isolate was ST75. PFGE showed that eight isolates with the same sequence type were of the same fingerprint types, which were of two closely-related subtypes.
In West China Hospital, some Acinetobacter baumannii isolates with reduced susceptibility to carbapenem carried bla(OXA-58). The major spread way of bla(OXA-58)-carrying isolates was clonal dissemination.
调查携带bla(OXA - 58)的鲍曼不动杆菌临床分离株的克隆相关性。
收集四川大学华西医院的非重复鲍曼不动杆菌分离株，通过recA测序进行验证。采用聚合酶链反应（PCR）检测获得性bla(OXA - 58)基因和天然bla(OXA51/66)基因。通过肠杆菌基因间重复共识聚合酶链反应（ERIC - PCR）、多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）对携带bla(OXA - 58)的鲍曼不动杆菌分离株进行菌株分型。
通过recA基因和bla(OXA - 51/66)检测共验证了115株鲍曼不动杆菌分离株。其中，观察到9株（7.8%）携带bla(OXA - 58)且对亚胺培南敏感性降低（MIC≥2mg/L）的分离株。9株携带bla(OXA - 58)的分离株的ERIC - PCR指纹高度相似。MLST显示8株分离株为ST95，1株为ST75。PFGE显示8株相同序列类型的分离株具有相同的指纹类型，属于两个密切相关的亚型。
在四川大学华西医院，一些对碳青霉烯类药物敏感性降低的鲍曼不动杆菌分离株携带bla(OXA - 58)。携带bla(OXA - 58)的分离株的主要传播方式是克隆传播。
