鲍曼不动杆菌分离株中OXA基因的基因组多样性研究。
Investigation on the genomic diversity of OXA from isolated Acinetobacter baumannii.
作者信息
Ma Zhen, Zhou Luqiu, Wang Hui, Luo Liangping
机构信息
Department of Medical Imaging Center, The First Affiliated Hospital of Jinan University Guangzhou 510632, China ; Department of Respiratory Diseases, Shenzhen Longgang Central Hospital Shenzhen 518116, China.
Department of Neurosurgery, Shenzhen Longgang Central Hospital Shenzhen 518116, China.
出版信息
Int J Clin Exp Med. 2015 Mar 15;8(3):4429-32. eCollection 2015.
We distinguished the four alleles of OXA subgroups from 138 strains of Acinetobacter baumannii using Polymerase Chain Reaction, and investigated distributions of OXA subgroups in clinical isolated strains. A total of 170 Acinetobacter baumannii were isolated from Shenzhen Longgang Central Hospital between 2010 and 2013. Amplification of OXA genes, blaOXA-23, blaOXA-24, blaOXA-51 and blaOXA-58, were performed by multiplex PCR. Multiplex PCR results showed, out of the 96 strains of Acinetobacter baumannii, 50 (52.08%) strains were positive for only blaOXA51 gene, and 46 (47.92%) showed positive for both blaOXA51 and blaOXA58 genes. Among 96 strains of Acinetobacter baumannii, 48 strains were resistant to carbapenems, and 48 strains were sensitivity to carbapenems. blaOXA51 and blaOXA58 showed resistant or sensitivity to carbapenems. In conclusion, we found that blaOXA-51 and blaOXA-5 were the main mechanisms of resistant or sensitivity to carbapenems.
我们使用聚合酶链反应从138株鲍曼不动杆菌中区分出OXA亚组的四个等位基因，并研究了OXA亚组在临床分离菌株中的分布情况。2010年至2013年期间，共从深圳龙岗中心医院分离出170株鲍曼不动杆菌。通过多重PCR对OXA基因blaOXA - 23、blaOXA - 24、blaOXA - 51和blaOXA - 58进行扩增。多重PCR结果显示，在96株鲍曼不动杆菌中，50株（52.08%）仅blaOXA51基因呈阳性，46株（47.92%）blaOXA51和blaOXA58基因均呈阳性。在96株鲍曼不动杆菌中，48株对碳青霉烯类耐药，48株对碳青霉烯类敏感。blaOXA51和blaOXA58对碳青霉烯类呈现耐药或敏感。总之，我们发现blaOXA - 51和blaOXA - 5是对碳青霉烯类耐药或敏感的主要机制。
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