Biomarkers. 1998;3(4-5):327-34. doi: 10.1080/135475098231138.
The goal of the present study was to measure the levels of 7-methylguanine and 7-(2- hydroxyethyl)guanine DNA adducts in human white blood cells in relation to smoking. DNA was isolated from samples of 11 smokers and eight non-smokers. The 32P-postlabelled 7-methylguanine and 7-(2-hydroxyethyl)guanine adducts were analysed by thin-layer chromatography (TLC) combined with a high pressure liquid chromatography (HPLC) assay. In smokers the mean 7-methylguanine and 7-(2-hydroxyethyl)guanine levels were 32.3 +/- 7.1 and 6.6 +/- 2.3 adducts per 108 nucleotides respectively. The corresponding values in non-smokers were 25.0 +/- 7.0 and 3.7 +/- 2.4 adducts per 108 nucleotides. There were significantly higher levels of 7-methylguanine and 7-(2-hydroxyethyl)guanine adducts in WBC in smokers than in non-smokers (p = 0.041; p = 0.018), respectively. A positive correlation between 7-methylguanine and 7-(2-hydroxyethyl)guanine levels was observed.
本研究的目的是测量与吸烟有关的人白细胞 DNA 中 7-甲基鸟嘌呤和 7-(2-羟乙基)鸟嘌呤 DNA 加合物的水平。从 11 名吸烟者和 8 名不吸烟者的样本中分离出 DNA。通过薄层层析（TLC）与高压液相色谱（HPLC）分析相结合，分析了 32P 标记的 7-甲基鸟嘌呤和 7-(2-羟乙基)鸟嘌呤加合物。在吸烟者中，7-甲基鸟嘌呤和 7-(2-羟乙基)鸟嘌呤的平均水平分别为每 108 个核苷酸 32.3 +/- 7.1 和 6.6 +/- 2.3 个加合物。非吸烟者相应的值分别为每 108 个核苷酸 25.0 +/- 7.0 和 3.7 +/- 2.4 个加合物。吸烟者白细胞中 7-甲基鸟嘌呤和 7-(2-羟乙基)鸟嘌呤加合物的水平明显高于非吸烟者（p = 0.041；p = 0.018）。观察到 7-甲基鸟嘌呤和 7-(2-羟乙基)鸟嘌呤水平之间存在正相关。
