Biomarkers. 1997;2(6):355-60. doi: 10.1080/135475097231445.
Quantification of 7 2 hydroxyethyl guanine 7 HEG adduct in DNA of livers and lymphocytes of male Sprague-Dawley rats exposed to 300 ppm ethene by inhalation 12 h a day for three consecutive days was performed to evaluate the potential of ethene to produce DNA adducts in these tissues. The persistence of 7 HEG in livers and lymphocytes was studied in rats sacrificed 0, 1, 5, and 20 days after the last exposure. DNA samples from control and treated animals were analysed for 7 HEG and 7 methylguanine 7 MG adducts by thin layer chromatography TLC combined with a high pressure liquid chromatography HPLC assay. After a 3 day exposure to ethene, 7 HEG accumulated to a similar extent in liver and lymphocytes, with the mean adduct level of 7.0 0.7 adducts per 107 nucleotides in liver and 7.4 0.7 adducts per 107 nucleotides in lymphocytes of rats sacrificed immediately after cessation of exposure. The approximate half life of 7 HEG was 5 days in liver and 3 days in lymphocytes, which is consistent with the loss of adduct primarily by spontaneous depurination. In addition, the background levels of 7 HEG and 7 MG were determined in the liver and lymphocytes from the control rats.
采用高效液相色谱法（HPLC）结合薄层层析（TLC）对 300ppm 乙烯吸入染毒，每天 12 小时，连续染毒 3 天的雄性 Sprague-Dawley 大鼠肝脏和淋巴细胞 DNA 中的 7-羟乙基鸟嘌呤（7-HEG）加合物进行定量分析，以评价乙烯在这些组织中产生 DNA 加合物的潜能。研究了大鼠末次染毒后 0、1、5 和 20 天处死时肝脏和淋巴细胞中 7-HEG 的残留情况。采用 TLC-HPLC 分析方法对对照组和染毒组大鼠的 DNA 样本进行 7-HEG 和 7-甲基鸟嘌呤（7-MG）加合物分析。染毒 3 天后，肝脏和淋巴细胞中 7-HEG 的蓄积程度相似，暴露停止后立即处死的大鼠肝脏和淋巴细胞中 7-HEG 的平均加合物水平分别为 7.00.7 个/107 核苷酸和 7.40.7 个/107 核苷酸。7-HEG 在肝脏和淋巴细胞中的近似半衰期分别为 5 天和 3 天，这与加合物主要通过自发脱嘌呤而丢失一致。此外，还测定了对照组大鼠肝脏和淋巴细胞中 7-HEG 和 7-MG 的背景水平。
