Materials Science and Engineering, CSIRO, Bayview Avenue, Clayton, VIC 3168, Australia.
Biomaterials. 2013 Nov;34(33):8361-9. doi: 10.1016/j.biomaterials.2013.07.042. Epub 2013 Jul 27.
In this work we have formulated Annexin V (ANX) decorated phosphatidylserine containing phytantriol (PSPhy) cubosomes to act as probes for the enhanced detection of apoptotic membranes in both model and in vitro cell systems. Small angle X-ray scattering (SAXS) and cryogenic-transmission electron microscopy (Cryo-TEM) indicated that ANX-containing PSPhy (ANX-PSPhy) cubosomes retain the Pn3m cubic symmetry and cubic phase nanoparticle characteristics of PSPhy cubosomes. The interaction of ANX-PSPhy cubosomes with apoptotic model and cellular membranes was also investigated using both quartz crystal microbalance with dissipation and confocal microscopy which confirmed that ANX-PSPhy cubosomes can selectively bind to apoptotic cells and model membranes. Neutron reflectometry has also been used to show strong binding of ANX-PSPhy cubosomes to a model apoptotic membrane, and in addition reveals changes in both the bilayer structure and in the internal structure of the cubosome in a region adjacent to the membrane as a result of material exchange. This material exchange between cubosome and apoptotic model bilayer was further demonstrated using Cryo-TEM. We have demonstrated that lipid bound protein, in this case Annexin V, can be used to target cubosome systems to biological surfaces in vitro.
在这项工作中，我们构建了含有磷脂酰丝氨酸的氢化植物三醇（PSPhy）立方脂质体，并用膜联蛋白 V（ANX）进行修饰，作为在模型和体外细胞系统中增强检测凋亡膜的探针。小角 X 射线散射（SAXS）和低温透射电子显微镜（Cryo-TEM）表明，含有 ANX 的 PSPhy（ANX-PSPhy）立方脂质体保留了 PSPhy 立方脂质体的 Pn3m 立方对称和立方相纳米颗粒特征。使用石英晶体微天平（QCM-D）和共聚焦显微镜也研究了 ANX-PSPhy 立方脂质体与凋亡模型和细胞膜的相互作用，证实了 ANX-PSPhy 立方脂质体可以选择性地与凋亡细胞和模型膜结合。中子反射率也被用来表明 ANX-PSPhy 立方脂质体与模型凋亡膜的强结合，此外，还揭示了由于物质交换，立方脂质体在膜附近区域的双层结构和内部结构发生变化。使用 Cryo-TEM 进一步证明了立方脂质体和凋亡模型双层之间的这种物质交换。我们已经证明，在这种情况下，结合在脂质上的蛋白质，即膜联蛋白 V，可以用于将立方脂质体系统靶向体外的生物表面。
