基本转录因子 3 参与胃癌的发生和发展。
Basic transcription factor 3 is involved in gastric cancer development and progression.
机构信息
Department of Anorectal Surgery, People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University, Changsha 410005, Hunan Province, China.
出版信息
World J Gastroenterol. 2013 Jul 28;19(28):4495-503. doi: 10.3748/wjg.v19.i28.4495.
AIM
To further analyse cancer involvement of basic transcription factor 3 (BTF3) after detection of its upregulation in gastric tumor samples.
METHODS
BTF3 transcription rates in human gastric tumor tissue samples (n = 20) and adjacent normal tissue (n = 18) specimens as well as in the gastric cancer cell lines AGS, SGC-7901, MKN-28, MKN-45 and MGC803 were analyzed via quantitative real-time polymerase chain reaction. The effect of stable BTF3 silencing via infection with a small interfering RNA (siRNA)-BTF3 expressing lentivirus on SGC-7901 cells was measured via Western blotting analysis, proliferation assays, cell cycle and apoptosis profiling by flow cytometry as well as colony forming assays with a Cellomic Assay System.
RESULTS
A significant higher expression of BTF3 mRNA was detected in tumors compared to normal gastric tissues (P < 0.01), especially in section tissues from female patients compared to male patients, and all tested gastric cancer cell lines expressed high levels of BTF3. From days 1 to 5, the relative proliferation rates of stable BTF3-siRNA transfected SGC7901 cells were 82%, 70%, 57%, 49% and 44% compared to the control, while the percentage of cells arrested in the G1 phase was significantly decreased (P = 0.000) and the percentages of cells in the S (P = 0.031) and G2/M (P = 0.027) phases were significantly increased. In addition, the colony forming tendency was significantly decreased (P = 0.014) and the apoptosis rate increased from 5.73% to 8.59% (P = 0.014) after BTF3 was silenced in SGC7901 cells.
CONCLUSION
BTF3 expression is associated with enhanced cell proliferation, reduced cell cycle regulation and apoptosis and its silencing decreased colony forming and proliferation of gastric cancer cells.
目的
在检测到基本转录因子 3（BTF3）在胃肿瘤样本中上调后，进一步分析其在癌症中的作用。
方法
通过定量实时聚合酶链反应分析 20 例人胃肿瘤组织样本和 18 例相邻正常组织样本以及胃癌细胞系 AGS、SGC-7901、MKN-28、MKN-45 和 MGC803 中 BTF3 的转录率。通过 Western blot 分析、增殖测定、细胞周期和凋亡分析以及使用 Cellomic 分析系统进行集落形成测定，来测量稳定沉默 BTF3 对 SGC-7901 细胞的影响。
结果
与正常胃组织相比，肿瘤中 BTF3 mRNA 的表达显著升高（P < 0.01），尤其是在女性患者的组织中，所有测试的胃癌细胞系均表达高水平的 BTF3。从第 1 天到第 5 天，与对照相比，稳定转染 BTF3-siRNA 的 SGC7901 细胞的相对增殖率分别为 82%、70%、57%、49%和 44%，而 G1 期细胞的比例显著降低（P = 0.000），S 期（P = 0.031）和 G2/M 期（P = 0.027）的细胞比例显著增加。此外，BTF3 沉默后，SGC7901 细胞的集落形成趋势显著降低（P = 0.014），凋亡率从 5.73%增加到 8.59%（P = 0.014）。
结论
BTF3 的表达与增强的细胞增殖、减少的细胞周期调节和凋亡有关，其沉默降低了胃癌细胞的集落形成和增殖。
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