Zhang Dianchang, Guo Huayang, Zhu Caiyan, Cui Ke, Zhang Nan, Jiang Shigui
Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , Guangzhou , China .
Mitochondrial DNA. 2015 Feb;26(1):149-50. doi: 10.3109/19401736.2013.819497. Epub 2013 Jul 31.
The complete mitochondrial genome of Cirrhinus molitorella was determined using the polymerase chain reaction. The complete mitochondrial DNA sequence is 16,602 bp in length. It consists of 13 protein-coding genes, 22 transfer RNA genes, 2 rRNA genes and 2 non-coding regions. Overall base composition of mitogenome is estimated to be 32.32% for A, 15.26% for G, 25.00% for T, 27.41% for C, respectively, with a high A + T content (57.32%). The control region contains a dinucleotide repeat motif, (TA)12, a termination-associated sequence and three conserved sequence blocks. The complete mitochondrial genome sequence of C. molitorella can provide a basic data for the studies on population structure, molecular systematic, stock evaluation and conservation genetics. It is also helpful to develop the rational management strategies for C. molitorella resource.
利用聚合酶链反应测定了鲮鱼的完整线粒体基因组。完整的线粒体DNA序列长度为16,602 bp。它由13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和2个非编码区组成。线粒体基因组的总体碱基组成估计分别为:A占32.32%、G占15.26%、T占25.00%、C占27.41%,A+T含量较高(57.32%)。控制区包含一个二核苷酸重复基序(TA)12、一个终止相关序列和三个保守序列块。鲮鱼完整的线粒体基因组序列可为种群结构、分子系统发育、种群评估和保护遗传学研究提供基础数据。这也有助于制定合理的鲮鱼资源管理策略。