Messier C, Durkin T, Mrabet O, Destrade C
Laboratoire de Psychophysiologie, Université de Bordeaux I, Talence, France.
Behav Brain Res. 1990 Jul 9;39(2):135-43. doi: 10.1016/0166-4328(90)90100-s.
The effect of a 3 g/kg glucose injection on the velocity of the sodium-dependent high-affinity choline uptake mechanism in the hippocampus was both measured in quiet control mice and in mice immediately after training in an operant bar pressing task. Glucose did not significantly change high-affinity choline uptake in resting animals. High-affinity choline uptake in the hippocampus was increased by training in the operant bar pressing task. Glucose significantly reduced the amplitude of the increase in high-affinity choline uptake observed in the trained animals. Similarly, a 3 g/kg glucose injection also attenuated the increase in high-affinity choline uptake observed in animals injected with 1 mg/kg scopolamine. Finally, a 3 g/kg glucose injection significantly attenuated the amnesia produced by a post-training 1 mg/kg scopolamine injection in mice trained for an operant bar pressing task. These results provide additional evidence for an action of glucose on hippocampal cholinergic activity under conditions of high acetylcholine demand. This action may be mediated via an increase in acetyl coenzyme A availability, one of the precursors of acetylcholine. This facilitative effect of glucose on hippocampal acetylcholine synthesis may constitute the physiological basis for its facilitative action on memory and its attenuation of scopolamine amnesia.
在安静的对照小鼠以及在操作性压杆任务训练后立即进行实验的小鼠中，均测量了3 g/kg葡萄糖注射对海马体中钠依赖性高亲和力胆碱摄取机制速度的影响。葡萄糖对静息动物的高亲和力胆碱摄取没有显著影响。操作性压杆任务训练可增加海马体中的高亲和力胆碱摄取。葡萄糖显著降低了训练动物中观察到的高亲和力胆碱摄取增加的幅度。同样，3 g/kg葡萄糖注射也减弱了注射1 mg/kg东莨菪碱的动物中观察到的高亲和力胆碱摄取的增加。最后，3 g/kg葡萄糖注射显著减轻了在操作性压杆任务训练的小鼠中，训练后注射1 mg/kg东莨菪碱所产生的失忆。这些结果为在高乙酰胆碱需求条件下葡萄糖对海马胆碱能活性的作用提供了额外证据。这种作用可能是通过增加乙酰辅酶A（乙酰胆碱的前体之一）的可用性来介导的。葡萄糖对海马乙酰胆碱合成的这种促进作用可能构成其对记忆的促进作用及其对东莨菪碱失忆的减轻作用的生理基础。
