Drug Applied Research Center & Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Future Oncol. 2013 Aug;9(8):1195-206. doi: 10.2217/fon.13.82.
Gelatin as a biodegradable, nontoxic and biocompatible natural protein is a good candidate for gene delivery. In this study, pDNA-loaded gelatin nanoparticles were prepared and characterized for the expression of the cytokine IL-12 and anti-tumor effects.
MATERIALS & METHODS: Gelatin-pUMVC3-hIL-12 nanoparticles were prepared by the ethanol precipitation technique and evaluated for physicochemical characteristics, cytotoxiciy and transfection efficiency.
The prepared particles were spherical in shape with sizes varying from 344.27 to 826.23 nm, ζ-potentials between -944 and -165 mV, and greater than 97% encapsulation efficiency. The particles were nontoxic to CT-26 carcinoma cells. The nanoparticles prepared using 0.5% gelatin solution (G14) with a mean particle size of 816.87 nm (polydispersity index = 0.56 ± 0.01) demonstrated maximum transfection efficiency with 2.5-times higher expression compared with the naked plasmid.
Gelatin-DNA nanoparticles using 0.5% gelatin solution had minimal cytotoxicity and can be used as a suitable candidate for further gene delivery studies and applications.
明胶作为一种可生物降解、无毒和生物相容的天然蛋白质，是基因传递的良好候选物。本研究制备了负载 pDNA 的明胶纳米粒，用于细胞因子 IL-12 的表达和抗肿瘤作用。
采用乙醇沉淀技术制备明胶-pUMVC3-hIL-12 纳米粒，并对其理化性质、细胞毒性和转染效率进行评价。
所制备的颗粒呈球形，粒径为 344.27-826.23nm，ζ-电位在-944 到-165mV 之间，包封率大于 97%。颗粒对 CT-26 癌细胞无毒性。使用 0.5%明胶溶液（G14）制备的平均粒径为 816.87nm（多分散指数=0.56±0.01）的纳米粒显示出最大的转染效率，与裸质粒相比表达水平提高了 2.5 倍。
使用 0.5%明胶溶液的明胶-DNA 纳米粒具有最小的细胞毒性，可作为进一步基因传递研究和应用的合适候选物。
