State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, Shanghai 201620, People's Republic of China.
Langmuir. 2013 Aug 13;29(32):10213-22. doi: 10.1021/la402118u. Epub 2013 Jul 31.
Many biomedical applications benefit from responsive polymer coatings. The properties of poly(dopamine) (PDA) films can be affected by codepositing dopamine (DA) with the temperature-responsive polymer poly(N-isopropylacrylamide) (pNiPAAm). We characterize the film assembly at 24 and 39 °C using DA and aminated or carboxylated pNiPAAm by a quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray photoelectron spectroscopy, UV-vis, ellipsometry, and atomic force microscopy. It was found that pNiPAAm with both types of end groups are incorporated into the films. We then identified a temperature-dependent adsorption behavior of proteins and liposomes to these PDA and pNiPAAm containing coatings by QCM-D and optical microscopy. Finally, a difference in myoblast cell response was found when these cells were allowed to adhere to these coatings. Taken together, these fundamental findings considerably broaden the potential biomedical applications of PDA films due to the added temperature responsiveness.
许多生物医学应用都受益于响应性聚合物涂层。聚多巴胺（PDA）薄膜的性能可以通过与温度响应性聚合物聚（N-异丙基丙烯酰胺）（pNiPAAm）共沉积多巴胺（DA）来影响。我们使用石英晶体微天平（QCM-D）、X 射线光电子能谱（XPS）、紫外-可见分光光度计（UV-vis）、椭圆光度法和原子力显微镜（AFM）在 24 和 39°C 下对含 DA 和氨基化或羧基化 pNiPAAm 的薄膜组装进行了表征。结果发现，具有这两种末端基团的 pNiPAAm 都被掺入到了薄膜中。然后，我们通过 QCM-D 和光学显微镜确定了蛋白质和脂质体在这些含有 PDA 和 pNiPAAm 的涂层上的温度依赖性吸附行为。最后，当这些细胞附着在这些涂层上时，我们发现成肌细胞的反应存在差异。总的来说，由于增加了温度响应性，这些基础发现极大地拓宽了 PDA 薄膜在生物医学应用中的潜力。
