State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University , Shanghai 201620, People's Republic of China.
J Phys Chem B. 2013 Sep 12;117(36):10504-12. doi: 10.1021/jp407106z. Epub 2013 Aug 29.
Mixed one-step poly(dopamine) (PDA)/highly branched poly(N-isopropylacrylamide) (pNiPAAm) coatings have been assembled and characterized by X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy, atomic force microscopy, and quartz crystal microbalance with dissipation monitoring (QCM-D) depending on the deposition temperature below and above the lower critical solution temperature (LCST) of the pNiPAAm. Mixed films were confirmed. The protein adsorption at 24 °C was found to be reduced with increasing amount of pNiPAAm in the mixed coatings, while there was no difference observed for proteins deposition at 39 °C. Further, the ability of these mixed coatings in comparison to the pure PDA and pNiPAAm films to serve as capping layer for surface-immobilized zwitterionic or positively charged liposomes has been assessed by QCM-D. The adhesion of hepatocytes, macrophages, and myoblast to these liposomes-containing hybrid coatings and the uptake of fluorescent lipids from the surface by the adhering cells depending on the capping layers were compared. The latter aspect was found to be dependent on the used capping layer and the type of liposome as carrier for the fluorescent lipid, with the highest uptake found for positive liposomes and pure pNiPAAm as capping layer. Taken together, the assembled hybrid coatings have the potential to be used as functional coatings toward surface-mediated drug delivery.
混合一步聚多巴胺 (PDA)/高支化聚 N-异丙基丙烯酰胺 (pNiPAAm) 涂层已通过 X 射线光电子能谱 (XPS)、紫外-可见光谱、原子力显微镜和石英晶体微天平耗散监测 (QCM-D) 进行组装和表征，具体取决于低于和高于 pNiPAAm 的低临界溶液温度 (LCST) 的沉积温度。证实了混合膜的存在。在 24°C 下，随着混合涂层中 pNiPAAm 含量的增加，蛋白质吸附量减少，而在 39°C 下，蛋白质沉积没有差异。此外，通过 QCM-D 评估了这些混合涂层与纯 PDA 和 pNiPAAm 薄膜相比作为表面固定两性离子或带正电脂质体的封盖层的能力。比较了含有这些脂质体的混合涂层对肝细胞、巨噬细胞和成肌细胞的黏附以及黏附细胞从表面摄取荧光脂质的能力，取决于封盖层。后一方面取决于所用的封盖层和作为荧光脂质载体的脂质体的类型，带有正电脂质体和纯 pNiPAAm 的封盖层的摄取量最高。总之，组装的混合涂层有可能用作表面介导药物输送的功能性涂层。
