Thanatvarakorn Ornnicha, Nakashima Syozi, Sadr Alireza, Prasansuttiporn Taweesak, Thitthaweerat Suppason, Tagami Junji
Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Dent Mater J. 2013;32(4):615-21. doi: 10.4012/dmj.2013-073.
This study aimed to evaluate the ability of a newly developed calcium-phosphate desensitizer in dentin permeability reduction and its integration with dentin surface before and after immersion in artificial saliva (AS) under two different dentin surface characteristics; with or without the collagen exposure.Humandentin discs treated by EDTA to expose collagen fibrils or EDTA/NaOCl to expose plain dentin surface were subjected to a calcium-phosphate desensitizer (Teethmate Desensitizer; TMD), while non-desensitizer treatment served as control. TMD application showed the occlusion in dentinal tubules and reduction in dentin permeability up to 92%, regardless of dentin surface characteristics. After AS immersion, permeability reduction percent (PR%) significantly increased in EDTA/NaOCl pretreatment (p<0.05). Newly-formed crystallites were observed on desensitizer treated dentin and EDTA/NaOCl pretreatment control group, whereas the crystallites did not exist on EDTA pretreatment control group. Ultrasonication revealed the integration of the calcium-phosphate rich layer of desensitizer on dentin surface after AS immersion.
本研究旨在评估一种新开发的磷酸钙脱敏剂在降低牙本质渗透性方面的能力，以及在两种不同牙本质表面特征（有或无胶原暴露）下，其在人工唾液（AS）中浸泡前后与牙本质表面的结合情况。用乙二胺四乙酸（EDTA）处理以暴露胶原纤维的人牙本质盘或用EDTA/次氯酸钠（NaOCl）处理以暴露普通牙本质表面的人牙本质盘，接受一种磷酸钙脱敏剂（Teethmate脱敏剂；TMD）处理，而未进行脱敏剂处理作为对照。无论牙本质表面特征如何，TMD的应用均显示牙本质小管被堵塞，牙本质渗透性降低达92%。在AS浸泡后，EDTA/NaOCl预处理组的渗透率降低百分比（PR%）显著增加（p<0.05）。在脱敏剂处理的牙本质和EDTA/NaOCl预处理对照组中观察到新形成的微晶，而在EDTA预处理对照组中不存在微晶。超声处理显示在AS浸泡后，脱敏剂富含磷酸钙的层与牙本质表面结合。
