Kobayashi K, Fukutani Y, Nakamura I, Kawasaki Y, Suzuki M, Yamaguchi N, Nakanishi I
Department of Neuropsychiatry School of Medicine, Kanazawa University, Japan.
No To Shinkei. 1990 Apr;42(4):345-51.
Morphometric study of neurons within the magnocellular basal forebrain system (MBFS) in the three normal controls and three cases with Alzheimer's disease (AD) was studied. Immunocytochemical staining using anti-acetylcholinesterase was performed to identify the measuring areas of the MBFS, and cross sectional areas of all neurons within the MBFS in one preparation was measured making use of cresyl violet staining. About 50% of the entire neuronal cells within the MBFS were decreased and case 2 had neurofibrillary tangles in the substantia innominata. In the septal nucleus neuronal cell depopulation was observed through the all range of the neuronal cell size, and in the diagonal band of Broca neuronal cells of which cross sectional areas were more than 200 microns2 were preferentially decreased and case 3 had inverse increase of the neurons of which cross sectional areas were less than 200 microns2. In the basal nucleus of Meynert in the substantia innominata neurons of which cross sectional areas were more than 250 microns2 were markedly decreased and neurons of which cross sectional areas less than 250 microns2 were well preserved. The large neurons within the basal nucleus and diagonal band of Broca were more affected in AD. In the septal nucleus and diagonal band it was suspected that non-cholinergic neurons were also decreased and these findings suggested that other series of monoamines also bore a relationship to the dementia and neuropsychological symptoms in AD.
对三名正常对照者和三名阿尔茨海默病（AD）患者的大细胞基底前脑系统（MBFS）内的神经元进行了形态计量学研究。使用抗乙酰胆碱酯酶进行免疫细胞化学染色以识别MBFS的测量区域，并利用甲酚紫染色测量一份标本中MBFS内所有神经元的横截面积。MBFS内约50%的神经元细胞减少，病例2在无名质中有神经原纤维缠结。在隔核中，观察到整个神经元细胞大小范围内的神经元细胞减少，在布罗卡斜带中，横截面积大于200平方微米的神经元细胞优先减少，病例3中横截面积小于200平方微米的神经元细胞反而增加。在无名质的迈内特基底核中，横截面积大于250平方微米的神经元明显减少，横截面积小于250平方微米的神经元保存良好。AD中，基底核和布罗卡斜带内的大神经元受影响更大。在隔核和斜带中，怀疑非胆碱能神经元也减少，这些发现表明其他系列的单胺也与AD中的痴呆和神经心理症状有关。
