Chemical Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras (IITM), Chennai 600036, India.
Org Biomol Chem. 2013 Sep 21;11(35):5853-65. doi: 10.1039/c3ob41244j.
A novel nucleic acid analogue called acyclic (S)-butyl nucleic acid (BuNA) composed of an acyclic backbone containing a phosphodiester linkage and bearing natural nucleobases was synthesized. Next, (S)-BuNA nucleotides were incorporated in DNA strands and their effect on duplex stability and changes in structural conformation were investigated. Circular dichroism (CD), UV-melting and non-denatured gel electrophoresis (native PAGE) studies revealed that (S)-BuNA is capable of making duplexes with its complementary strands and integration of (S)-BuNA nucleotides into DNA duplex does not alter the B-type-helical structure of the duplex. Furthermore, (S)-BuNA oligonucleotides and (S)-BuNA substituted DNA strands were studied as primer extensions by DNA polymerases. This study revealed that the acyclic scaffold is tolerated by enzymes and is therefore to some extent biocompatible.
一种新型的核酸类似物，称为无环（S）-丁基核酸（BuNA），由无环骨架组成，含有磷酸二酯键，并带有天然核碱基。接下来，将（S）-BuNA 核苷酸掺入 DNA 链中，并研究它们对双链稳定性和结构构象变化的影响。圆二色性（CD）、紫外熔融和非变性凝胶电泳（native PAGE）研究表明，（S）-BuNA 能够与互补链形成双链，并且将（S）-BuNA 核苷酸整合到 DNA 双链中不会改变双链的 B 型螺旋结构。此外，还研究了（S）-BuNA 寡核苷酸和（S）-BuNA 取代的 DNA 链作为 DNA 聚合酶的引物延伸。这项研究表明，无环支架可被酶耐受，因此在某种程度上是生物相容的。
