Narayan K S, Moress E A, Chatham J C, Barker P B
Huntington Medical Research Institutes, Pasadena, CA 91101.
NMR Biomed. 1990 Feb;3(1):23-6. doi: 10.1002/nbm.1940030104.
A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate-fre medium designed for maintenance of high cell viability. Intracellular pH and beta-NTP/Pi ratios, studied in three cell types, remained stable over a 16-20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.
本文描述了一种用于培养哺乳动物细胞进行长期³¹P磁共振波谱分析的简单技术。细胞被包裹在海藻酸钙凝胶中，并用一种专为维持高细胞活力而设计的新型无磷酸盐培养基进行灌注。在三种细胞类型中研究的细胞内pH值和β-NTP/Pi比率，在16 - 20小时的核磁共振监测期内保持稳定。所描述的技术可能有助于监测多种细胞类型的细胞代谢。
