Wu Yuan-Jie, Fang Zhao-Hui, Zheng Shu-Guo, Wu Yuan-Bo, Wang Zheng, Lu Ming-an
Department of Basic Theory of Traditional Chinese Medicine, Anhui College of Traditional Chinese Medicine, Hefei 230038, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 May;33(5):641-5.
To explore effects of exercise combined Danzhi Jiangtang Capsule (DJC) on the protein expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase p22phox in pancreatic tissues of diabetic rats.
Sixty male Wistar rats were injected with low dose of streptozotocin and fed with high fat diet to establish a diabetic rat model. The levels of p22phox and 8-hydroxy-2-de-oxyguanosine (8-OHdG) protein in pancreatic tissues were detected by immunohistochemical method, and the level of p22phox protein was also detected by Western blot in the normal group, the model group, the excise group, the DJC group, and the DJC +excise group, respectively.
The expression levels of p22phox and 8-OHdG protein in pancreatic tissues were significantly higher in the model group than in the normal group (P <0.01). p22phox and 8-OHdG were mainly expressed in the cytoplasm of pancreatic cells. After administration of exercise or DJC, the expression lev- els of p22phox or 8-OHdG protein in pancreatic tissues decreased significantly (P <0. 01). Exercise combined DJC had synergistic effects in decreasing expressions of p22phox and 8-OHdG (P <0.05).
Exercise, DJC, and exercise combined DJC could protect islet beta cells by decreasing the expression of NADPH oxidase in beta cells and reducing sources of oxidative stress.
探讨运动联合丹蛭降糖胶囊（DJC）对糖尿病大鼠胰腺组织中烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶p22phox蛋白表达的影响。
60只雄性Wistar大鼠注射低剂量链脲佐菌素并给予高脂饮食以建立糖尿病大鼠模型。采用免疫组化法检测胰腺组织中p22phox和8-羟基-2'-脱氧鸟苷（8-OHdG）蛋白水平，并用蛋白质免疫印迹法分别检测正常组、模型组、运动组、DJC组和DJC+运动组中p22phox蛋白水平。
模型组胰腺组织中p22phox和8-OHdG蛋白表达水平显著高于正常组（P<0.01）。p22phox和8-OHdG主要在胰腺细胞胞质中表达。运动或DJC给药后，胰腺组织中p22phox或8-OHdG蛋白表达水平显著降低（P<0.01）。运动联合DJC在降低p22phox和8-OHdG表达方面具有协同作用（P<0.05）。
运动、DJC以及运动联合DJC可通过降低β细胞中NADPH氧化酶的表达和减少氧化应激来源来保护胰岛β细胞。
