Shi Qian-Wei, Li Shu-Guang, Li Jun, Ling Chang-quan
Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 May;33(5):659-63.
To observe in vitro and in vivo effects of triptolide on growth inhibition and apoptosis of osteosarcoma cells, and to further explore its correlated molecular mechanisms.
The growth inhibition effects of triptolide on osteosarcoma cells were detected using MTT assay. The apoptosis was detected using flow cytometry.The protein expressions of associated signals were detected using Western blot. The in vivo anti-osteosarcoma effects of triptolide were verified in osteosarcoma nude mice. The in vivo associated protein expressions were detected using immunohistochemistry (IHC).
Triptolide could significantly inhibit the proliferation of various osteosarcoma cells. Besides, it could induce their apoptosis. Triptolide triggered the mitochondrial dependent apoptotic pathway, significantly inhibited the in vivo growth of osteosarcoma cells, and caused in vivo apoptosis.
Triptolide induced apoptosis of osteosarcoma cells partially through activating mitochondria associated apoptosis signal pathway. Triptolide also induced apoptosis of osteosarcoma cells and inhibited their in vivo growth in nude mice.
观察雷公藤甲素对骨肉瘤细胞生长抑制和凋亡的体外及体内作用，并进一步探讨其相关分子机制。
采用MTT法检测雷公藤甲素对骨肉瘤细胞的生长抑制作用。采用流式细胞术检测细胞凋亡情况。采用蛋白质免疫印迹法检测相关信号蛋白的表达。在骨肉瘤裸鼠体内验证雷公藤甲素的抗骨肉瘤作用。采用免疫组织化学法检测体内相关蛋白的表达。
雷公藤甲素可显著抑制多种骨肉瘤细胞的增殖。此外，它还能诱导细胞凋亡。雷公藤甲素触发线粒体依赖性凋亡途径，显著抑制骨肉瘤细胞的体内生长，并导致体内细胞凋亡。
雷公藤甲素部分通过激活线粒体相关凋亡信号通路诱导骨肉瘤细胞凋亡。雷公藤甲素还可诱导骨肉瘤细胞凋亡并抑制其在裸鼠体内的生长。
