Programa de Pós-Graduação em Ciência de Alimentos, Universidade Estadual de Maringá, Avenida Colombo 5790, Maringá 87020-900, Paraná, Brazil.
J Food Prot. 2013 Aug;76(8):1408-13. doi: 10.4315/0362-028X.JFP-13-020.
This study evaluated the adhesion and biofilm formation of Alicyclobacillus acidoterrestris on industrial orange juice processing equipment and the bactericidal efficacy of peracetic acid, sodium hypochlorite, and quaternary ammonia after biofilm formation. The efficacy of these sanitizers against the spores of this microorganism was also evaluated. Stainless steel and nylon surfaces exhibited higher cell adhesion levels than did polyvinyl chloride surfaces. Peracetic acid was the most effective in removing biofilms from all surfaces (P < 0.05) and also reduced bacterial counts by 3 log CFU/cm² on the surface of polyvinyl chloride, but the other sanitizers also reduced the bacterial counts by 2 log CFU/cm². Quaternary ammonia exhibited the optimal minimum sporicidal concentration, preventing spore germination after only 15 s of contact at a concentration of 82 ppm. The flow cytometry results indicated that the spores and cells had low incidences of plasma membrane lysis after treatment with sanitizer, suggesting that lysis is not the principal mode of action for these sanitizers on A. acidoterrestris.
本研究评估了耐热性环状酸芽孢杆菌在工业橙汁加工设备上的黏附与生物膜形成情况，以及生物膜形成后过氧乙酸、次氯酸钠和季铵盐的杀菌效果。还评估了这些消毒剂对该微生物孢子的杀菌效果。不锈钢和尼龙表面的细胞黏附水平高于聚氯乙烯表面。过氧乙酸对所有表面的生物膜去除效果最显著（P＜0.05），并且在聚氯乙烯表面减少了 3 个对数 CFU/cm²的细菌数量，但其他消毒剂也减少了 2 个对数 CFU/cm²的细菌数量。季铵盐表现出最佳的最低杀孢子浓度，在 82ppm 浓度下仅接触 15s 即可防止孢子萌发。流式细胞术结果表明，消毒剂处理后孢子和细胞的质膜裂解发生率较低，表明质膜裂解不是这些消毒剂对 A. acidoterrestris 作用的主要方式。
