Centre Technique de la Conservation des Produits Agricoles, Site Agroparc, ZA de l'aéroport, BP 21 203, F-84 911 Avignon cedex 9, France.
J Food Prot. 2012 Feb;75(2):371-5. doi: 10.4315/0362-028X.JFP-11-329.
The aim of this study was to determine the inactivation effect of industrial formulations of peracetic acid biocides on bacterial spores adhering to stainless steel surfaces. A standardized protocol was used to validate biocide activity against spores in suspension. To validate sporicidal activity under practical conditions, we developed an additional protocol to simulate industrial sanitization of stainless steel surfaces with a foam sanitizer. Spores of three spore-forming bacteria, Clostridium sporogenes PA3679, Geobacillus stearothermophilus, and Moorella thermoacetica/thermoautotrophica, were sprayed onto stainless steel as bioaerosols. Sporicidal activity was high against the C. sporogenes spore suspension, with more than 5 log CFU ml(-1) destroyed at all liquid biocide contact times. Sporicidal activity also was high against G. stearothermophilus and M. thermoacetica/thermoautotrophica spores after 30 min of contact, but we found no population reduction at the 5-min contact time for the highest sporicide concentration tested. The foam biocide effectively inactivated C. sporogenes spores adhered to stainless steel but had a reduced decontamination effect on other species. For G. stearothermophilus spores, sanitization with the foam sporicide was more efficient on horizontal steel than on vertical steel, but foam sanitization was ineffective against M. thermoacetica/thermoautotrophica whatever the position. These results highlight that decontamination efficiency may differ depending on whether spores are suspended in an aqueous solution or adhered to a stainless steel surface. Biocide efficiency must be validated using relevant protocols and bacteria representative of the microbiological challenges and issues affecting each food industry.
本研究旨在确定工业过氧乙酸消毒剂制剂对附着在不锈钢表面的细菌孢子的灭活效果。采用标准化方案验证悬浮液中孢子的消毒剂活性。为了在实际条件下验证杀菌活性，我们开发了一种额外的方案，以模拟使用泡沫消毒剂对不锈钢表面进行工业消毒。将三种产孢细菌（凝结芽孢杆菌 PA3679、嗜热脂肪芽孢杆菌和热乙酸/热自养莫拉氏菌）的孢子作为生物气溶胶喷洒到不锈钢上。消毒剂对 C. sporogenes 孢子悬浮液的杀菌活性很高，所有液体消毒剂接触时间均能破坏超过 5 个对数 CFU ml(-1)。G. stearothermophilus 和 M. thermoacetica/thermoautotrophica 孢子在接触 30 分钟后也具有较高的杀菌活性，但在测试的最高杀孢子剂浓度下，5 分钟接触时间没有观察到种群减少。泡沫消毒剂可有效灭活附着在不锈钢上的 C. sporogenes 孢子，但对其他物种的消毒效果降低。对于嗜热脂肪芽孢杆菌孢子，泡沫杀孢子剂对水平钢的消毒效果优于垂直钢，但泡沫消毒对莫拉氏菌无效，无论位置如何。这些结果表明，消毒效率可能因孢子是悬浮在水溶液中还是附着在不锈钢表面而有所不同。必须使用相关方案和代表影响每个食品行业的微生物挑战和问题的细菌来验证杀生物剂的效率。
