Department of Chemistry, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Langmuir. 2013 Aug 27;29(34):10850-8. doi: 10.1021/la402007d. Epub 2013 Aug 16.
Iron oxide nanoparticles (IONPs) and their surface modifications with therapeutic or diagnostic (theranostic, TN) agents are of great interest. Here we present a novel one-pot synthesis of a versatile general TN precursor (aminosilane-coated IONPs [IONP-Sil(NH2)]) with surface amine groups. Surface functional group conversion to carboxylic acid was accomplished by conjugating poly(ethylene glycol) diacid to IONP-Sil(NH2). The NPs were characterized using powder X-ray diffraction (XRD), transmission electron microscopy (TEM), high-resolution TEM, selected area electron diffraction (SAED), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FT-IR) spectroscopy. Biocompatibility and cell uptake profile of the nanoparticles were evaluated in-vitro using cultured liver cells (HepG2). Oleylamine (hydrophobic) and bovine serum albumin (BSA) as model drugs were attached to IONP-Sil-PEG(COOH). The ability of IONP-Sil(NH2) to bind small interfering RNA (siRNA) is also shown.
氧化铁纳米粒子（IONPs）及其与治疗或诊断（治疗诊断，TN）剂的表面修饰受到了极大的关注。在这里，我们提出了一种新颖的一锅合成方法，用于制备具有表面氨基的多功能通用 TN 前体（氨基硅烷包覆的 IONPs [IONP-Sil(NH2)]）。通过将聚乙二醇二羧酸偶联到 IONP-Sil(NH2)上，实现了表面功能基团向羧酸的转化。使用粉末 X 射线衍射（XRD）、透射电子显微镜（TEM）、高分辨率 TEM、选区电子衍射（SAED）、X 射线光电子能谱（XPS）和傅里叶变换红外（FT-IR）光谱对 NPs 进行了表征。使用培养的肝细胞（HepG2）在体外评估了纳米粒子的生物相容性和细胞摄取特性。油胺（疏水性）和牛血清白蛋白（BSA）作为模型药物被连接到 IONP-Sil-PEG(COOH)上。还展示了 IONP-Sil(NH2)结合小干扰 RNA（siRNA）的能力。
