Salehiabar Marziyeh, Nosrati Hamed, Davaran Soodabeh, Danafar Hossein, Manjili Hamidreza Kheiri
Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of pharmaceutical biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Drug Res (Stuttg). 2018 May;68(5):280-285. doi: 10.1055/s-0043-120197. Epub 2017 Oct 16.
Natural L-aspartic acid coated iron oxide magnetic nanoparticles (Asp@IONPs) were prepared by a one pot, in-situ and green co-precipitation method in an aqueous medium. Functionalized iron oxide magnetic nanoparticles (IONPs) were characterized by Vibrating Sample Magnetometer (VSM), X-ray diffraction (XRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) techniques. Cellular toxicity of IONPs was also investigated on HEK-293 cell lines. The results showed that the zeta potential of Asp@IONPs was about -21.1 mV and the average size was 17.80±3.09 nm. Cell toxicity results show that as prepared IONPs are biocompatible. Asp@IONPs show the possibility of using these nanoparticles in the development of in vitro and in vivo biomedical fields due to do not possess a toxic effect, good ζ-potential and related small and narrow size distribution.
天然L-天冬氨酸包覆的氧化铁磁性纳米颗粒（Asp@IONPs）通过一锅法、原位和绿色共沉淀法在水介质中制备。通过振动样品磁强计（VSM）、X射线衍射（XRD）、差示扫描量热法（DSC）、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）技术对功能化的氧化铁磁性纳米颗粒（IONPs）进行了表征。还研究了IONPs对HEK-293细胞系的细胞毒性。结果表明，Asp@IONPs的zeta电位约为-21.1 mV，平均粒径为17.80±3.09 nm。细胞毒性结果表明，所制备的IONPs具有生物相容性。由于不具有毒性作用、良好的ζ电位以及相关的小尺寸和窄尺寸分布，Asp@IONPs显示出在体外和体内生物医学领域开发中使用这些纳米颗粒的可能性。
