Guichard M, Lespinasse F, Estelin R, Gerbaulet A, Haie C, Lartigau E, Malaise E P, Micheau C, Prade M, Richard J M
Laboratoire de Radiobiologie Cellulaire (Unité Inserm 247), Institut Gustave-Roussy, Villejuif, France.
Br J Radiol. 1990 Jul;63(751):557-61. doi: 10.1259/0007-1285-63-751-557.
There is evidence that some human tumours could be treated with a combination of buthionine sulfoximine and hypoxic cell sensitizers. However, clinical application of this technique requires a prior knowledge of the level of non-protein bound sulfhydryl (NPSH) compounds in these tumours. The present study provides data on the levels of glutathione (GSH) and cysteine (CYS) in human tumour biopsies from the cervix and from the head and neck. The NPSH compounds were measured by high performance liquid chromatography. The median GSH values were 20.5 nmol/mg protein (cervix) and 23 nmol/mg protein (head and neck) while the median CYS values were 4.4 (cervix) and 4.2 nmol/mg protein (head and neck). The values varied widely from one patient to another.
有证据表明，一些人类肿瘤可以用丁硫氨酸亚砜胺和乏氧细胞增敏剂联合治疗。然而，这项技术的临床应用需要事先了解这些肿瘤中非蛋白结合巯基（NPSH）化合物的水平。本研究提供了来自宫颈以及头颈部的人类肿瘤活检组织中谷胱甘肽（GSH）和半胱氨酸（CYS）水平的数据。通过高效液相色谱法测定NPSH化合物。GSH的中位数分别为20.5 nmol/mg蛋白质（宫颈）和23 nmol/mg蛋白质（头颈部），而CYS的中位数分别为4.4（宫颈）和4.2 nmol/mg蛋白质（头颈部）。这些值在不同患者之间差异很大。
