Loro Giovanna, Costa Alex
Department of Biology, University of Padova, 35131 Padova, Italy.
Cold Spring Harb Protoc. 2013 Aug 1;2013(8):781-5. doi: 10.1101/pdb.prot073049.
Here we report a method for analyzing mitochondrial Ca(2+) dynamics in plant root cells by means of the newly generated cameleon probe 4mt-YC3.6. The use of plants expressing both nuclear- and mitochondrial-targeted cameleon, along with the resolution of confocal laser scanning microscopy (CLSM), allow simultaneous recordings of mitochondrial and nuclear Ca(2+) dynamics within the same cell.
在此，我们报告一种利用新生成的钙指示剂探针4mt-YC3.6分析植物根细胞中线粒体Ca(2+)动态变化的方法。使用同时表达定位于细胞核和线粒体的钙指示剂的植物，结合共聚焦激光扫描显微镜（CLSM）的分辨率，能够在同一细胞内同时记录线粒体和细胞核的Ca(2+)动态变化。
