Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Analyst. 2013 Oct 7;138(19):5819-25. doi: 10.1039/c3an00758h.
A novel, rapid and highly sensitive fluorescence turn-on assay for the detection of biothiols (glutathione, and L-cysteine), histidine and threonine was developed. Water-soluble CuInS2 ternary quantum dots (QDs) capped by mercaptopropionic acid (MPA) were directly synthesized in aqueous solution, and then functionalized using tyrosine molecules to form tyrosine-functionalized CuInS2 QDs (T-CuInS2 QDs). The fluorescence of T-CuInS2 QDs would decrease in the presence of Cu(2+) due to the coordination effect of phenolic hydroxyls of the tyrosine molecules. Subsequently, the addition of biothiols (glutathione and L-cysteine), histidine or threonine could turn on the fluorescence of the T-CuInS2 QDs-Cu(2+) system due to their strong affinity for Cu(2+). The proposed method was simple in design and fast in operation, and it was applied for the detection of glutathione, L-cysteine, histidine, and threonine in human serum samples with satisfactory results.
一种用于检测生物硫醇（谷胱甘肽和 L-半胱氨酸）、组氨酸和苏氨酸的新型、快速和高灵敏度荧光开启分析方法被开发出来。水溶性巯基丙酸（MPA）包覆的 CuInS2 三元量子点（QDs）在水溶液中直接合成，然后用酪氨酸分子进行功能化，形成酪氨酸功能化的 CuInS2 QDs（T-CuInS2 QDs）。由于酪氨酸分子的酚羟基的配位作用，T-CuInS2 QDs 的荧光会在存在 Cu(2+)时降低。随后，由于生物硫醇（谷胱甘肽和 L-半胱氨酸）、组氨酸或苏氨酸与 Cu(2+)具有很强的亲和力，因此可以打开 T-CuInS2 QDs-Cu(2+)体系的荧光。所提出的方法设计简单，操作快速，用于检测人血清样品中的谷胱甘肽、L-半胱氨酸、组氨酸和苏氨酸，结果令人满意。
