Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Talanta. 2013 Nov 15;116:870-5. doi: 10.1016/j.talanta.2013.07.073. Epub 2013 Aug 9.
In this paper, a novel near-infrared fluorescence probe for the determination of 2,4,6-trinitrophenol (TNP) was developed based on bovine serum albumin (BSA) coated CuInS2 quantum dot (QD). Water-soluble CuInS2 QDs were directly synthesized in aqueous solution with mercaptopropionic acid (MPA) as stablizers, and it was coated by BSA via an amide link interacting with carboxyl of the MPA-capped CuInS2 QDs. The obtained BSA coated CuInS2 QDs (BSA-CuInS2 QDs) can bind TNP in water via the acid-base pairing interaction between electron-rich amino groups of BSA and electron-deficient nitroaromatic rings, and leading the fluorescence quenching of BSA-CuInS2 QDs. The quenched fluorescence intensity of BSA-CuInS2 QDs was proportional to the concentration of TNP in the concentration ranges from 50 nmol/L to 3.0 μmol/L. The detection limit (LOD) for TNP was 28 nmol/L. The developed fluorescence probe showed a excellent resistant to interference of metal ions such as K(+), Na(+), Zn(2+), Cu(2+), Hg(2+), and Pb(2+). The developed biosensor was applied to the determination of TNP in spiked water samples with satisfactory results.
本文基于牛血清白蛋白（BSA）包覆的 CuInS2 量子点（QD），开发了一种用于测定 2,4,6-三硝基苯酚（TNP）的新型近红外荧光探针。水溶性 CuInS2 QD 采用巯基丙酸（MPA）作为稳定剂直接在水溶液中合成，通过与 MPA 封端的 CuInS2 QD 上的羧基相互作用的酰胺键被 BSA 包覆。所得的 BSA 包覆的 CuInS2 QD（BSA-CuInS2 QD）可以在水中通过 BSA 中富电子的氨基与缺电子的硝基芳烃环之间的酸碱配对相互作用与 TNP 结合，导致 BSA-CuInS2 QD 的荧光猝灭。BSA-CuInS2 QD 的猝灭荧光强度与 TNP 的浓度在 50 nmol/L 至 3.0 μmol/L 的浓度范围内成正比。TNP 的检测限（LOD）为 28 nmol/L。所开发的荧光探针对 K(+)、Na(+)、Zn(2+)、Cu(2+)、Hg(2+)和 Pb(2+)等金属离子的干扰具有优异的抗性。该生物传感器已成功应用于加标水样中 TNP 的测定。
