Department of Life Sciences, Chonbuk National University, Jeonju-si 561-756, Republic of Korea.
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4508-4514. doi: 10.1099/ijs.0.053439-0. Epub 2013 Aug 1.
Two strains, designated GCR0103(T) and GYR3121(T), were isolated from seawater of the Saemangeum Embankment in Jeollabuk-do, Korea. The cells of the two strains were Gram-reaction-negative and non-motile, and formed multicellular filaments. The colonies of the two strains were pink-pigmented and able to grow at 15-37 °C (optimum 25 °C) on R2A and NA medium. Strains GCR0103(T) and GYR3121(T) grew at pH 6.5-10 (optimum pH 7.5) and pH 5.5-9.5 (optimum pH 7.5), and within NaCl ranges of 0-0.4% and 0-1%, respectively. The polar lipid profiles of the two strains contained phosphatidylethanolamine, five unknown aminolipids, an unknown phospholipid and four or five unknown lipids. The DNA G+C contents of strains GCR0103(T) and GYR3121(T) were 56.0 and 54.5 mol%, respectively. The respiratory quinone detected in both strains was MK-7. The 16S rRNA gene sequence similarity between GCR0103(T) and GYR3121(T) was 95.5 %. The 16S rRNA gene sequence similarities of the two strains to closely related reference strains were less than 89 %. Phylogenetic analysis based on 16S rRNA genes showed that GCR0103(T) and GYR3121(T) formed a distinct phyletic line in the family Cytophagaceae. On the basis of the phenotypic, chemotaxonomic and phylogenetic properties, strains GCR0103(T) and GYR3121(T) represent two novel species in a new genus within the family Cytophagaceae, for which the names Nibrella saemangeumensis gen. nov., sp. nov. and Nibrella viscosa sp. nov. are proposed. The type strain of Nibrella saemangeumensis is GCR0103(T) ( = KACC 16453(T) = JCM 17927(T)) and the type strain of Nibrella viscosa is GYR3121(T) ( = KACC 16447(T) = JCM 17925(T)).
两株菌，分别命名为 GCR0103(T)和 GYR3121(T)，从韩国全罗北道的咸镜南道堤岸的海水中分离得到。两株菌的细胞革兰氏反应阴性，无动力，形成多细胞丝。两株菌的菌落呈粉红色，能够在 R2A 和 NA 培养基上于 15-37°C（最佳温度 25°C）生长。GCR0103(T)和 GYR3121(T)在 pH 6.5-10（最佳 pH 7.5）和 pH 5.5-9.5（最佳 pH 7.5）范围内生长，盐度分别为 0-0.4%和 0-1%。两株菌的极性脂谱均含有磷脂酰乙醇胺、五种未知的氨基脂、一种未知的磷脂和四到五种未知的脂类。GCR0103(T)和 GYR3121(T)的 DNA G+C 含量分别为 56.0 和 54.5 mol%。两株菌均检测到 MK-7 为呼吸醌。GCR0103(T)和 GYR3121(T)的 16S rRNA 基因序列相似度为 95.5%。两株菌与密切相关的参考菌株的 16S rRNA 基因序列相似度均小于 89%。基于 16S rRNA 基因的系统发育分析表明，GCR0103(T)和 GYR3121(T)在 Cytophagaceae 科中形成了一个独特的进化枝。根据表型、化学分类和系统发育特征，GCR0103(T)和 GYR3121(T)代表 Cytophagaceae 科中新属的两个新种，分别命名为 Nibrella saemangeumensis gen. nov.，sp. nov.和 Nibrella viscosa sp. nov.。Nibrella saemangeumensis 的模式菌株为 GCR0103(T)（=KACC 16453(T)=JCM 17927(T)），Nibrella viscosa 的模式菌株为 GYR3121(T)（=KACC 16447(T)=JCM 17925(T)）。
