Department of Life Sciences, Chonbuk National University, Jeonju-si, 561-756, Republic of Korea.
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4568-4573. doi: 10.1099/ijs.0.051870-0. Epub 2013 Aug 1.
Two Gram-reaction-negative, rod-shaped, non-motile and red-pink-pigmented bacterial strains, designated GYR3077(T) and GSR0100(T), were isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do, South Korea, and were characterized using a polyphasic approach. 16S rRNA genes of strains GYR3077(T) and GSR0100(T) exhibited sequence similarities of 95.9 % to Hymenobacter deserti ZLB-3(T) and 96.6 % to Hymenobacter soli PB17(T), respectively, and indicated that these isolates belonged to the phylum Bacteroidetes. The major cellular fatty acids present in the two isolates were iso-C15 : 0, C16 : 1ω5c, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The major respiratory quinone and polyamine patterns were menaquinone-7 and sym-homospermidine, characteristic of the genus Hymenobacter. Flexirubin-type pigments were absent in both strains. The DNA G+C contents of strains GYR3077(T) and GSR0100(T) were 60.2 mol% and 61.9 mol%, respectively. The major polar lipid of strains GYR3077(T) and GSR0100(T) was phosphatidylethanolamine. Based on the morphological and physiological properties, strains GYR3077(T) and GSR0100(T) were considered to represent two novel species of the genus Hymenobacter, for which the names Hymenobacter koreensis sp. nov. (type strain GYR3077(T) = KACC 16451(T) = JCM 17924(T)) and Hymenobacter saemangeumensis sp. nov. (type strain GSR0100(T) = KACC 16452(T) = JCM 17923(T)) are proposed.
两株革兰氏反应阴性、杆状、非运动性和粉红色着色的细菌菌株，命名为 GYR3077(T) 和 GSR0100(T)，从韩国全罗北道的咸镜南道三陟市的 Mangyung 河口的一个水样中分离得到，并采用多相方法进行了表征。菌株 GYR3077(T) 和 GSR0100(T) 的 16S rRNA 基因序列相似性分别为 95.9%与 Hymenobacter deserti ZLB-3(T)和 96.6%与 Hymenobacter soli PB17(T)，表明这些分离株属于厚壁菌门。这两个分离株中存在的主要细胞脂肪酸为 iso-C15:0、C16:1ω5c、总和特征 4（iso-C17:1 I 和/或 anteiso-C17:1 B）和总和特征 3（C16:1ω7c 和/或 C16:1ω6c）。两个菌株的主要呼吸醌和聚胺模式为menaquinone-7 和 sym-homospermidine，这是 Hymenobacter 属的特征。两个菌株均不含 flexirubin 型色素。菌株 GYR3077(T) 和 GSR0100(T) 的 DNA G+C 含量分别为 60.2 mol%和 61.9 mol%。菌株 GYR3077(T) 和 GSR0100(T) 的主要极性脂为磷脂酰乙醇胺。基于形态和生理特性，菌株 GYR3077(T) 和 GSR0100(T) 被认为代表了 Hymenobacter 属的两个新种，分别命名为 Hymenobacter koreensis sp. nov.（模式菌株 GYR3077(T) = KACC 16451(T) = JCM 17924(T)）和 Hymenobacter saemangeumensis sp. nov.（模式菌株 GSR0100(T) = KACC 16452(T) = JCM 17923(T)）。
