Vitteková Miroslava, Dragúňová Jana, Kabát Peter, Božiková Martina, Bakoš Dušan, Koller Ján
Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
Cell Tissue Bank. 2014 Sep;15(3):345-55. doi: 10.1007/s10561-013-9390-0. Epub 2013 Aug 2.
The preparation and study of three-dimensional functional skin substitutes has been the focus of intense research for several decades. Dermal substitutes are now commonly used in medical practice for a variety of applications. Here, we assess the toxicity of seven selected acellular dermal matrix materials to establish their potential for use in future three-dimensional skin substitute studies. The cytotoxicity of acellular dermis (of Allo- and Xenograft origin) prepared in our lab and biomaterials based on collagen and hyaluronic acid (Coladerm H and Coladerm H-L) were compared to that seen in three commercially available products (Xe-Derma, AlloDerm and Xeno-Impl). Murine fibroblasts NIH-3T3 and human dermal fibroblasts were used in cytotoxicity tests, with any resultant cytotoxic effects caused by the seven tested dermal scaffolds visualised using an inverted microscope system and confirmed in parallel using colorimetric MTT cell proliferation assays. While most of the dermal substitutes did not demonstrate a cytotoxic effect on our two cell types, Xeno and Xeno-Impl scaffolds clearly did. The cytotoxic effect of acellular Xeno dermal matrix could essentially be removed through a regime of multiple washes, but we were unable to remove the cytotoxic effect of Xeno-Impl. Thus, Xeno-Impl alone has been excluded from our future work on preparation of 3D skin substitutes.
几十年来，三维功能性皮肤替代物的制备和研究一直是深入研究的焦点。真皮替代物目前在医学实践中被广泛用于各种应用。在此，我们评估了七种选定的脱细胞真皮基质材料的毒性，以确定它们在未来三维皮肤替代物研究中的应用潜力。将我们实验室制备的（同种异体和异种移植来源的）脱细胞真皮以及基于胶原蛋白和透明质酸的生物材料（Coladerm H和Coladerm H-L）的细胞毒性与三种市售产品（Xe-Derma、AlloDerm和Xeno-Impl）进行了比较。在细胞毒性测试中使用了小鼠成纤维细胞NIH-3T3和人真皮成纤维细胞，使用倒置显微镜系统观察七种测试真皮支架产生的任何细胞毒性作用，并同时使用比色MTT细胞增殖测定法进行确认。虽然大多数真皮替代物对我们的两种细胞类型没有显示出细胞毒性作用，但Xeno和Xeno-Impl支架显然有。脱细胞异种真皮基质的细胞毒性作用基本上可以通过多次洗涤去除，但我们无法去除Xeno-Impl的细胞毒性作用。因此，在我们未来制备三维皮肤替代物的工作中，仅将Xeno-Impl排除在外。
