College of Food Science and Engineering, Liaoning Medical University, Jinzhou, 121000, China,
Biotechnol Lett. 2013 Dec;35(12):2099-103. doi: 10.1007/s10529-013-1305-4. Epub 2013 Aug 2.
Although most bacteria produce fatty acids (FA), few secrete free FAs into the culture media. Over-expression of two FA thioesterases, TesA and AtFatA, facilitated both total and FFA production in a recombinant strain of Escherichia coli. When these thioesterases were expressed in a fadD and fadL double-deletion strain, a further enhancement of FFA secretion was observed. These results support a simple diffusion mechanism for FA transport. In addition, the ATP-binding cassette transporter protein, MsbA, also increased the concentration of FFAs in the culture. The final strain produced 110 mg FFA/l, about 33 % of the total FAs being produced. Our findings support a diffusion mechanism for FA transport.
虽然大多数细菌都能产生脂肪酸（FA），但很少有细菌将游离脂肪酸（FFA）分泌到培养基中。过表达两种 FA 硫酯酶 TesA 和 AtFatA ，可促进大肠杆菌重组菌株的总脂肪酸和 FFA 的产生。当这些硫酯酶在 fadD 和 fadL 双缺失菌株中表达时，FFA 的分泌进一步增强。这些结果支持 FA 转运的简单扩散机制。此外，ATP 结合盒转运蛋白 MsbA 也增加了培养基中 FFAs 的浓度。最终的菌株产生了 110mgFFA/L，约占总 FFA 的 33%。我们的研究结果支持 FA 转运的扩散机制。
