Laboratorio de Oftalmología Experimental, Facultad de Medicina, Universidad de Murcia, Campus de Espinardo, Murcia, Spain.
Invest Ophthalmol Vis Sci. 2013 Aug 28;54(8):5888-900. doi: 10.1167/iovs.13-12643.
To investigate the spatiotemporal relationship between rod and cone degeneration in the P23H-1 rat.
Control Sprague-Dawley (SD) and P23H-1 rats of ages ranging from P30 to P365 were used. Retinas were processed for whole mounts or cross sections and rods and cones were immunodetected. We used newly developed image analysis techniques to quantify the total population of L/M cones (the most abundant cones in the rat) and analyzed the rings of rod-cone degeneration.
In P23H-1 rats, rod degeneration occurs rapidly: first the rod outer segment shortens, at P30 there is extensive rod loss, and by P180 rod loss is almost complete except for the most peripheral retina. The numbers of L/M cones are, at all postnatal ages, lower in P23H-1 rats than in control SD rats, and decrease significantly with age (by P180). Rod and cone degeneration is spatiotemporally related and occurs in rings that appear already at P90 and spread throughout the entire retina. At P180, the rings of rod-cone degeneration are more abundant in the equatorial retina and are larger in the dorsal retina.
This work describes for the first time that in the P23H-1 rat, rod and cone degeneration is spatiotemporally related and occurs in rings. Cone loss follows rod loss and starts very soon, even before P30, the first age analyzed here. The characteristics of the rings suggest that secondary cone degeneration is influenced by retinal position and/or other intrinsic or extrinsic factors.
研究 P23H-1 大鼠中视杆和视锥变性的时空关系。
使用 P30 至 P365 日龄的对照 Sprague-Dawley（SD）大鼠和 P23H-1 大鼠。对视网膜进行全铺片或横切片处理，并对视杆和视锥进行免疫检测。我们使用新开发的图像分析技术来定量测定 L/M 视锥（大鼠中最丰富的视锥）的总群体，并分析视杆-视锥变性环。
在 P23H-1 大鼠中，视杆变性发生迅速：首先是视杆外节缩短，在 P30 时出现广泛的视杆丧失，到 P180 时除了最周边的视网膜外，视杆丧失几乎完全。在所有出生后年龄，P23H-1 大鼠的 L/M 视锥数量均低于对照 SD 大鼠，并且随着年龄的增长显著减少（到 P180 时）。视杆和视锥变性在时空上是相关的，发生在已经在 P90 时出现的环中，并在整个视网膜中扩散。在 P180 时，赤道视网膜中的视杆-视锥变性环更丰富，并且在背侧视网膜中更大。
本工作首次描述了在 P23H-1 大鼠中，视杆和视锥变性在时空上是相关的，并且发生在环中。视锥丧失紧随视杆丧失之后，甚至在 P30 之前就已经开始，这是此处分析的最早年龄。环的特征表明，次级视锥变性受视网膜位置和/或其他内在或外在因素的影响。
