Department of Chemical Engineering, University of Seoul, Seoulsiripdaero 163, Dongdaemun-gu, Seoul 130-743, Republic of Korea.
J Biomed Nanotechnol. 2013 Jul;9(7):1245-9. doi: 10.1166/jbn.2013.1538.
Recently, DNA has been used to guide the self-assembly of functional materials. Based on programmability of DNA, branched DNA nanostructures were created and precisely labeled with quantum dots and gold nanoparticles. The precise molecular recognition of DNA allows the precise design and synthesis of multifunctional hybrid materials by building DNA nanostructures. The first step of obtaining multi-functionality is to precisely label function materials on DNA building block or branched DNA before construction of complicated nanostructures. In this paper, we introduce the two routes to label functional nanoparticle on DNA structure. Quantum dots were successfully conjugated on branched DNA by streptavidin-biotin interaction and gold nanoparticles were attached on the DNA nanostructure by hybridization.
最近，DNA 已被用于指导功能材料的自组装。基于 DNA 的可编程性，构建了分支 DNA 纳米结构，并使用量子点和金纳米粒子进行了精确标记。DNA 的精确分子识别允许通过构建 DNA 纳米结构来精确设计和合成多功能杂化材料。获得多功能性的第一步是在构建复杂的纳米结构之前，在 DNA 构建块或分支 DNA 上精确标记功能材料。在本文中，我们介绍了两种在 DNA 结构上标记功能纳米粒子的方法。通过链霉亲和素-生物素相互作用成功地将量子点偶联到分支 DNA 上，并且通过杂交将金纳米粒子附着到 DNA 纳米结构上。
