Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, Kerala, India.
J Biomed Nanotechnol. 2013 Jul;9(7):1299-305. doi: 10.1166/jbn.2013.1630.
This work explains about the development of a unique tubular scaffold for vascular tissue engineering. The inner layer/layers was made up of aligned poly (lactic acid) (PLA) nano fibers and outer layers were composed of random multiscale fibers of poly(caprolactone) (PCL)/PLA providing larger pores for Smooth Muscle Cell (SMC) penetration. The fabricated scaffolds were characterized by SEM. Cell attachment and infiltration studies using SMCs on the multiscale fibers were characterized by SEM and confocal microscopy. Blood compatibility of the scaffold was analysed by haemolysis-coagulation assays, platelet activation studies and the effect of material/fiber alignment on the morphological stability of Red Blood Cells (RBCs) were evaluated using SEM. Since this hierarchically designed tubular scaffold closely mimics the morphology of native vessel, this could be a better candidate for vascular tissue engineering.
这项工作介绍了一种用于血管组织工程的独特管状支架的开发。内层/层由定向排列的聚乳酸（PLA）纳米纤维组成，外层由聚己内酯（PCL）/PLA 的随机多尺度纤维组成，为平滑肌细胞（SMC）渗透提供更大的孔。通过 SEM 对制备的支架进行了表征。使用 SMC 对多尺度纤维进行细胞附着和渗透研究，通过 SEM 和共聚焦显微镜进行了表征。通过溶血-凝血测定分析支架的血液相容性，通过血小板激活研究评估材料/纤维取向对红细胞（RBC）形态稳定性的影响，使用 SEM 进行评估。由于这种分层设计的管状支架紧密模拟了天然血管的形态，因此它可能是血管组织工程的更好候选者。
