Vaz C M, van Tuijl S, Bouten C V C, Baaijens F P T
Department of Biomedical Engineering, Division of Biomechanics and Tissue Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
Acta Biomater. 2005 Sep;1(5):575-82. doi: 10.1016/j.actbio.2005.06.006. Epub 2005 Jul 26.
Aiming to develop a scaffold architecture mimicking morphological and mechanically that of a blood vessel, a sequential multi-layering electrospinning (ME) was performed on a rotating mandrel-type collector. A bi-layered tubular scaffold composed of a stiff and oriented PLA outside fibrous layer and a pliable and randomly oriented PCL fibrous inner layer (PLA/PCL) was fabricated. Control over the level of fibre orientation of the different layers was achieved through the rotation speed of the collector. The structural and mechanical properties of the scaffolds were examined using scanning electron microscopy (SEM) and tensile testing. To assess their capability to support cell attachment, proliferation and migration, 3T3 mouse fibroblasts and later human venous myofibroblasts (HVS) were cultured, expanded and seeded on the scaffolds. In both cases, the cell-polymer constructs were cultured under static conditions for up to 4 weeks. Environmental-scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), histological examination and biochemical assays for cell proliferation (DNA) and extracellular matrix production (collagen and glycosaminoglycans) were performed. The findings suggest the feasibility of ME to design scaffolds with a hierarchical organization through a layer-by-layer process and control over fibre orientation. The resulting scaffolds achieved the desirable levels of pliability (elastic up to 10% strain) and proved to be capable to promote cell growth and proliferation. The electrospun PLA/PCL bi-layered tube presents appropriate characteristics to be considered a candidate scaffold for blood vessel tissue engineering.
为了开发一种在形态和机械性能上模仿血管的支架结构，在旋转心轴式收集器上进行了连续多层静电纺丝（ME）。制备了一种双层管状支架，其外层由坚硬且取向的聚乳酸（PLA）纤维层组成，内层由柔韧且随机取向的聚己内酯（PCL）纤维层组成（PLA/PCL）。通过收集器的转速来控制不同层的纤维取向水平。使用扫描电子显微镜（SEM）和拉伸试验对支架的结构和力学性能进行了检测。为了评估它们支持细胞附着、增殖和迁移的能力，培养、扩增了3T3小鼠成纤维细胞以及后来的人静脉肌成纤维细胞（HVS），并将其接种到支架上。在这两种情况下，细胞-聚合物构建体在静态条件下培养长达4周。进行了环境扫描电子显微镜（SEM）、共聚焦激光扫描显微镜（CLSM）、组织学检查以及细胞增殖（DNA）和细胞外基质产生（胶原蛋白和糖胺聚糖）的生化分析。研究结果表明，通过逐层工艺和对纤维取向的控制，ME设计具有分层结构的支架是可行的。所得支架达到了理想的柔韧性水平（在应变高达10%时具有弹性），并证明能够促进细胞生长和增殖。静电纺丝的PLA/PCL双层管具有适当的特性，可被视为血管组织工程的候选支架。
