Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University, Chongqing 400044, China.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):3753-9. doi: 10.1016/j.msec.2013.05.003. Epub 2013 May 8.
To investigate the influence of magnetized hydroxyapatite on the growth and differentiation of osteoblasts, hydroxyapatite (HA) and magnetized hydroxyapatite (mHA) were synthesized and characterized. The cell viability, differentiation, and morphologies of osteoblasts were investigated in vitro, respectively. The results showed that compared to HA, cells cultured with mHA had better cell viability, and both HA and mHA were beneficial to the early differentiation of osteoblasts. Furthermore, the interaction mechanism between mHA and osteoblasts was elucidated using a molecular dynamics simulation. The simulation results indicated that when cultured with osteoblasts, HA adsorbed bovine serum protein onto its surface from the medium immediately, which was beneficial to the adhesion and proliferation of osteoblasts. The main driving force for the adsorption of bovine serum was the electronic properties of HA crystal faces. The (211) crystal face of HA had the highest electron density among its all crystal faces, thus mainly contributing to the protein adsorption of HA. Nevertheless, the (211) crystal face of mHA still had a relatively higher electron density than that of HA, thus possessing better protein adsorption than that of HA, and in turn promoting the biological functions of osteoblasts.
为了研究磁羟基磷灰石对成骨细胞生长和分化的影响，我们合成并表征了羟基磷灰石（HA）和磁羟基磷灰石（mHA）。分别在体外研究了成骨细胞的细胞活力、分化和形态。结果表明，与 HA 相比，培养在 mHA 上的细胞具有更好的细胞活力，并且 HA 和 mHA 都有利于成骨细胞的早期分化。此外，我们还使用分子动力学模拟阐明了 mHA 与成骨细胞之间的相互作用机制。模拟结果表明，当与成骨细胞共培养时，HA 立即从培养基中吸附牛血清蛋白到其表面，这有利于成骨细胞的黏附和增殖。吸附牛血清的主要驱动力是 HA 晶面的电子特性。HA 的（211）晶面在其所有晶面中具有最高的电子密度，因此主要有助于 HA 的蛋白质吸附。然而，mHA 的（211）晶面的电子密度仍然比 HA 高，因此具有比 HA 更好的蛋白质吸附能力，从而促进成骨细胞的生物学功能。
