Department of Microbiology and Immunology, University of Rochester, Rochester, NY 14642, USA.
Bioorg Med Chem Lett. 2013 Sep 15;23(18):5199-202. doi: 10.1016/j.bmcl.2013.06.097. Epub 2013 Jul 8.
Cationic amyloid fibrils, including the Semen Enhancer of Virus Infection (SEVI), have recently been described in human semen. Simple methods for quantitating these fibrils are needed to improve our understanding of their biological function. We performed high-throughput screening to identify molecules that bind SEVI, and identified a small molecule (8E2), that fluoresced brightly in the presence of SEVI and other cationic fibrils. 8E2 bound SEVI with almost 40-fold greater affinity than thioflavin-T, and could efficiently detect high molecular weight fibrils in human seminal fluid.
阳离子淀粉样纤维，包括病毒感染增强因子（SEVI），最近在人类精液中被描述。需要简单的定量这些纤维的方法来提高我们对其生物学功能的理解。我们进行了高通量筛选，以鉴定与 SEVI 结合的分子，并鉴定出一种小分子（8E2），在存在 SEVI 和其他阳离子纤维的情况下发出明亮的荧光。8E2 与 SEVI 的结合亲和力比噻唑橙 T 高近 40 倍，并且能够有效地检测人精液中的高分子量纤维。
