Department of Physiological Sciences, Faculty of Science, Stellenbosch University, Stellenbosch Private Bag X1 Matieland, Stellenbosh, 7602, South Africa.
Central Analytical Facility, Stellenbosch University, Stellenbosch Private Bag X1 Matieland, Stellenbosh, 7602, South Africa.
Cardiovasc Diabetol. 2017 Nov 2;16(1):141. doi: 10.1186/s12933-017-0624-5.
We have previously shown that many chronic, inflammatory diseases are accompanied, and possibly partly caused or exacerbated, by various coagulopathies, manifested as anomalous clots in the form of 'dense matted deposits'. More recently, we have shown that these clots can be amyloid in nature, and that the plasma of healthy controls can be induced to form such clots by the addition of tiny amounts of bacterial lipopolysaccharide or lipoteichoic acid. Type 2 diabetes (T2D) is also accompanied by raised levels of LPS.
We use superresolution and confocal microscopies to investigate the amyloid nature of clots from healthy and T2D individuals.
We show here, with the established stain thioflavin T and the novel stains Amytracker™ 480 and 680, that the clotting of plasma from type 2 diabetics is also amyloid in nature, and that this may be prevented by the addition of suitable concentrations of LPS-binding protein.
This implies strongly that there is indeed a microbial component to the development of type 2 diabetes, and suggests that LBP might be used as treatment for it and its sequelae.
我们之前已经表明，许多慢性炎症性疾病伴有各种凝血异常，表现为“密集交织沉积物”形式的异常血栓，可能是由这些凝血异常引起或加重的。最近，我们发现这些血栓可能是淀粉样的，并且健康对照者的血浆可以通过添加少量细菌脂多糖或脂磷壁酸来诱导形成这种血栓。2 型糖尿病（T2D）也伴随着 LPS 水平升高。
我们使用超分辨率和共聚焦显微镜来研究来自健康和 T2D 个体的血栓的淀粉样性质。
我们在这里使用已建立的染色试剂硫黄素 T 和新型染色试剂 Amytracker™480 和 680 表明，2 型糖尿病患者血浆的凝结也是淀粉样的，并且可以通过添加适当浓度的 LPS 结合蛋白来预防。
这强烈表明，2 型糖尿病的发生确实存在微生物成分，并表明 LBP 可能可用于治疗 2 型糖尿病及其后果。
