Laboratório de Transmissores de Leishmanioses, Setor de Entomologia Médica e Forense, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Brazil; Doutoranda do Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, RJ, Brazil.
Acta Trop. 2013 Dec;128(3):522-7. doi: 10.1016/j.actatropica.2013.07.016. Epub 2013 Jul 30.
Peckia (Euboettcheria) collusor (Diptera: Sarcophagidae) is a necrophagous insect and represents an important role in forensic entomology, as it is always collected at animal/human corpses. Immature sarcophagids are extremely similar in morphological aspects and can be found colonizing both fresh and mummified bodies. Flesh fly larvae are reported to cause accidental myiasis. In the Neotropical regions little is known about the ultrastructure of immature stages of Sarcophagidae. Scanning electron microscopy gives detailed information on the morphological characters, which can help identify the immature forms of the flies. The aim of this study is to identify larvae and puparia of P. (E.) collusor using this methodology. The integument with net-like patches and the shape and disposition of intersegmental spines of the larval instars can be used to distinguish this flesh fly larva. The number of spiracular ramifications is variable at larval instars. The puparia are very similar to third instar larvae, except for the cephalic structures that are retracted. In conclusion, scanning electron microscopy could help entomologists to identify the immature forms of P. (E.) collusor in forensic investigations and consequently serves as a tool in crime scene investigations.
(伊氏)啄果葬甲(双翅目：葬甲科)是一种腐食性昆虫，在法医昆虫学中起着重要作用，因为它总是在动物/人类尸体上被收集到。未成熟的葬甲在形态上非常相似，可以在新鲜和木乃伊化的尸体上发现它们的存在。报道称肉蝇幼虫会引起意外蝇蛆病。在新热带地区，关于葬甲科未成熟阶段的超微结构知之甚少。扫描电子显微镜可以提供关于形态特征的详细信息，这有助于识别蝇类的未成熟形态。本研究旨在使用这种方法来鉴定(伊氏)啄果葬甲的幼虫和蛹。幼虫各龄的表皮具有网状斑块，体节间刺的形状和排列可以用来区分这种肉蝇幼虫。幼虫的气门分支数量是可变的。蛹与第三龄幼虫非常相似，除了头部结构缩回。总之，扫描电子显微镜可以帮助昆虫学家在法医学调查中识别(伊氏)啄果葬甲的未成熟形态，因此可以作为犯罪现场调查的工具。
