Department of Pharmaceutics, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, Iran.
Carbohydr Polym. 2013 Sep 12;97(2):277-83. doi: 10.1016/j.carbpol.2013.04.098. Epub 2013 May 9.
The nanoparticles of oligochitosan-water soluble tragacanth (OCH-WST) as novel gene carriers have been prepared and their transfection efficiency has been investigated on Hela and HepG2 cell lines. Different OCH:WST weight ratios were prepared to obtain particles with low size distribution and high surface charge, and also in range of below 200 nm. Nanoparticles with 132.5 ± 6.77 nm size, polydispersity index 1.92 ± 0.061, surface charge 30.45 ± 1.84 and spherical morphology, have been chosen as gene carrier. Nanoparticle-DNA complexes (nanoplexes) showed better transfection efficiency in both Hela and HepG2 cells than chitosan polyplexes, with 1.26 × 10(6) versus 9.05 × 10(5) and 7.76 × 10(5) versus 2.16 × 10(5), respectively. Higher transfection efficiency of nanoplexes could be attributed to their weaker complexation. Decreasing of transfection in presence of galactose in HepG2 cells, indicated receptor mediated endocytosis of nanoplexes. These properties all together, make OCH-WST nanoparticles as potential gene carrier for active gene delivery into cells containing sugar receptors.
以寡糖壳聚糖-水溶性刺槐豆胶（OCH-WST）纳米粒为新型基因载体进行了制备，并在 Hela 和 HepG2 细胞系上研究了其转染效率。 制备了不同的 OCH：WST 重量比，以获得具有低粒径分布和高表面电荷的颗粒，并且粒径也在 200nm 以下。 选择粒径为 132.5 ± 6.77nm、多分散指数为 1.92 ± 0.061、表面电荷为 30.45 ± 1.84、呈球形的纳米粒作为基因载体。 与壳聚糖多聚物相比，纳米粒-DNA 复合物（纳米复合物）在 Hela 和 HepG2 细胞中均显示出更好的转染效率，分别为 1.26×10(6)与 9.05×10(5)和 7.76×10(5)与 2.16×10(5)。 纳米复合物具有较弱的复合物形成能力，从而导致更高的转染效率。 在 HepG2 细胞中存在半乳糖时转染效率降低，表明纳米复合物通过受体介导的内吞作用进入细胞。 所有这些特性使 OCH-WST 纳米粒成为具有糖受体的细胞中活性基因递送至的潜在基因载体。
