School of Life Sciences and National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
Carbohydr Polym. 2013 Sep 12;97(2):731-5. doi: 10.1016/j.carbpol.2013.05.051. Epub 2013 May 27.
Burdock fructooligosaccharide (BFO) isolated from the root tissue of Arctium lappa is a reserve carbohydrate that can induce resistance against a number of plant diseases. Stomatal closure is a part of plant innate immune response to restrict bacterial invasion. In this study, the effects of BFO on stomata movement in Pisum sativum and the possible mechanisms were studied with abscisic acid (ABA) as a positive control. The results showed that BFO could induce stomatal closure accompanied by ROS and NO production, as is the case with ABA. BFO-induced stomatal closure was inhibited by pre-treatment with L-NAME (N(G)-nitro-L-arginine methyl ester, hydrochloride; nitric oxide synthase inhibitor) and catalase (hydrogen peroxide scavenger). Exogenous catalase completely restricted BFO-induced production of ROS and NO in guard cells. In contrast, L-NAME prevented the rise in NO levels but only partially restricted the ROS production. These results indicate that BFO-induced stomatal closure is mediated by ROS and ROS-dependent NO production.
牛蒡低聚果糖（BFO）是从牛蒡根组织中分离得到的一种储备性碳水化合物，能够诱导植物抵抗多种病害。气孔关闭是植物固有免疫反应的一部分，可以限制细菌的入侵。在这项研究中，以脱落酸（ABA）作为阳性对照，研究了 BFO 对豌豆气孔运动的影响及其可能的机制。结果表明，BFO 能够诱导气孔关闭，同时伴随着 ROS 和 NO 的产生，这与 ABA 的情况相同。用 L-NAME（N（G）-硝基-L-精氨酸甲酯盐酸盐；一氧化氮合酶抑制剂）和过氧化氢酶（过氧化氢清除剂）预处理可以抑制 BFO 诱导的气孔关闭。外源过氧化氢酶完全抑制了保卫细胞中 BFO 诱导的 ROS 和 NO 的产生。相比之下，L-NAME 阻止了 NO 水平的升高，但仅部分抑制了 ROS 的产生。这些结果表明，BFO 诱导的气孔关闭是由 ROS 和 ROS 依赖性的 NO 产生介导的。
